In Vivo Epithelial Metabolic Imaging Using a Topical Fluorescent Glucose Analog

Paula Villarreal, Rahul Pal, Gracie Vargas

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The demanding metabolic needs of cancer cells are met by aerobic glycolysis. While whole-body PET imaging methods exist for evaluating this metabolic response, these are not ideal for local, more detailed regions such as mucosal surfaces. Fluorescence imaging of glucose analogs with similarities to radiolabeled deoxyglucose used in PET, namely, fluorescent 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-NBDG), offers such an alternative, particularly as this glucose analog may be delivered by local topical delivery. In this chapter, methods for in vivo epithelial imaging in a preclinical hamster model for oral cancer and oral epithelial dysplasia are described. Outlined are methods for preparation and in vivo delivery of 2-NBDG by topical application to the oral mucosa followed by fluorescence imaging to compare fluorescence responses between neoplasia and control mucosa or to monitor changes in fluorescence signal with time in both groups.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages21-31
Number of pages11
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2126
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Epithelial cancer
  • Epithelial dysplasia
  • Fluorescence
  • Glycolysis
  • Intravital
  • Metabolism
  • Optical Imaging
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'In Vivo Epithelial Metabolic Imaging Using a Topical Fluorescent Glucose Analog'. Together they form a unique fingerprint.

  • Cite this

    Villarreal, P., Pal, R., & Vargas, G. (2020). In Vivo Epithelial Metabolic Imaging Using a Topical Fluorescent Glucose Analog. In Methods in Molecular Biology (pp. 21-31). (Methods in Molecular Biology; Vol. 2126). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0364-2_3