@inbook{1ba014e4b016409bb53075b6d50c3aaa,
title = "In Vivo Epithelial Metabolic Imaging Using a Topical Fluorescent Glucose Analog",
abstract = "The demanding metabolic needs of cancer cells are met by aerobic glycolysis. While whole-body PET imaging methods exist for evaluating this metabolic response, these are not ideal for local, more detailed regions such as mucosal surfaces. Fluorescence imaging of glucose analogs with similarities to radiolabeled deoxyglucose used in PET, namely, fluorescent 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-d-glucose (2-NBDG), offers such an alternative, particularly as this glucose analog may be delivered by local topical delivery. In this chapter, methods for in vivo epithelial imaging in a preclinical hamster model for oral cancer and oral epithelial dysplasia are described. Outlined are methods for preparation and in vivo delivery of 2-NBDG by topical application to the oral mucosa followed by fluorescence imaging to compare fluorescence responses between neoplasia and control mucosa or to monitor changes in fluorescence signal with time in both groups.",
keywords = "Epithelial cancer, Epithelial dysplasia, Fluorescence, Glycolysis, Intravital, Metabolism, Optical Imaging, Oral squamous cell carcinoma",
author = "Paula Villarreal and Rahul Pal and Gracie Vargas",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-0716-0364-2_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "21--31",
booktitle = "Methods in Molecular Biology",
}