In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models

Frances Valencia, Ronald L. Veselenak, Nigel Bourne

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Both the guinea pig and mouse are important animal models for the study of genital herpes. The murine model has been used extensively to evaluate vaccines and antiviral agents by measuring the incidence of infection and the magnitude of viral replication; however, this model is limited with regard to distinguishing between candidate vaccines or treatments. In contrast, the guinea pig closely mimics human infection and provides an excellent model of both primary and recurrent genital herpes disease. This animal model is especially important in the study of viral transmission through the evaluation of latent viral reactivation and virus shedding into the genital tract. Here, we describe methodologies to determine viral infection, severity of primary disease, and quantification of primary viral replication in the genital tract for both the guinea pig and murine models of genital herpes. Additionally, we detail the evaluation of the onset of primary disease and progression to the day of death in the mouse model. Further, we summarize methods to assess the frequency of recurrences, frequency and magnitude of virus shedding, and latent viral load in the sensory nerve ganglia of the guinea pig.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages315-326
Number of pages12
Volume1030
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1030
ISSN (Print)10643745

Fingerprint

Herpes Genitalis
Virus Shedding
Antiviral Agents
Guinea Pigs
Virus Diseases
Vaccines
Animal Models
Sensory Ganglia
Viral Load
Disease Progression
Recurrence
Incidence
Infection

Keywords

  • Animal model
  • Genital herpes
  • Guinea pig
  • Herpes simplex viruses
  • HSV-2
  • Mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Valencia, F., Veselenak, R. L., & Bourne, N. (2013). In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. In Methods in Molecular Biology (Vol. 1030, pp. 315-326). (Methods in Molecular Biology; Vol. 1030). https://doi.org/10.1007/978-1-62703-484-5_24

In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. / Valencia, Frances; Veselenak, Ronald L.; Bourne, Nigel.

Methods in Molecular Biology. Vol. 1030 2013. p. 315-326 (Methods in Molecular Biology; Vol. 1030).

Research output: Chapter in Book/Report/Conference proceedingChapter

Valencia, F, Veselenak, RL & Bourne, N 2013, In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. in Methods in Molecular Biology. vol. 1030, Methods in Molecular Biology, vol. 1030, pp. 315-326. https://doi.org/10.1007/978-1-62703-484-5_24
Valencia F, Veselenak RL, Bourne N. In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. In Methods in Molecular Biology. Vol. 1030. 2013. p. 315-326. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-484-5_24
Valencia, Frances ; Veselenak, Ronald L. ; Bourne, Nigel. / In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. Methods in Molecular Biology. Vol. 1030 2013. pp. 315-326 (Methods in Molecular Biology).
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