In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models

Frances Valencia, Ronald L. Veselenak, Nigel Bourne

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

Both the guinea pig and mouse are important animal models for the study of genital herpes. The murine model has been used extensively to evaluate vaccines and antiviral agents by measuring the incidence of infection and the magnitude of viral replication; however, this model is limited with regard to distinguishing between candidate vaccines or treatments. In contrast, the guinea pig closely mimics human infection and provides an excellent model of both primary and recurrent genital herpes disease. This animal model is especially important in the study of viral transmission through the evaluation of latent viral reactivation and virus shedding into the genital tract. Here, we describe methodologies to determine viral infection, severity of primary disease, and quantification of primary viral replication in the genital tract for both the guinea pig and murine models of genital herpes. Additionally, we detail the evaluation of the onset of primary disease and progression to the day of death in the mouse model. Further, we summarize methods to assess the frequency of recurrences, frequency and magnitude of virus shedding, and latent viral load in the sensory nerve ganglia of the guinea pig.

Original languageEnglish (US)
Title of host publicationAntiviral Methods and Protocols
Pages315-326
Number of pages12
DOIs
StatePublished - Jul 18 2013

Publication series

NameMethods in Molecular Biology
Volume1030
ISSN (Print)1064-3745

Keywords

  • Animal model
  • Genital herpes
  • Guinea pig
  • HSV-2
  • Herpes simplex viruses
  • Mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Valencia, F., Veselenak, R. L., & Bourne, N. (2013). In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models. In Antiviral Methods and Protocols (pp. 315-326). (Methods in Molecular Biology; Vol. 1030). https://doi.org/10.1007/978-1-62703-484-5_24