@inbook{f9500440f40f44f68ab4a2b370de9c9b,
title = "In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models",
abstract = "In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.",
keywords = "Fluorescence, In vivo imaging, Luminescence, Recombinant Nipah virus, Reporter genes, Rodent animal model",
author = "Kendra Johnson and Terry Juelich and Jennifer Smith and Benhur Lee and Freiberg, {Alexander N.}",
note = "Funding Information: This work was supported by National Institutes of Health (grant numbers R33 AI102267 to A.N.F. and B.L., RO1 AI123449 to B.L., and R21 AI138233 to A.N.F.) and by the University of Texas Medical Branch Department of Pathology start-up funds (to A.N.F.). Publisher Copyright: {\textcopyright} 2023, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3283-3_11",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "149--157",
booktitle = "Methods in Molecular Biology",
}