In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models

Kendra Johnson, Terry Juelich, Jennifer Smith, Benhur Lee, Alexander N. Freiberg

Research output: Chapter in Book/Report/Conference proceedingChapter


In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
StatePublished - 2023
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Fluorescence
  • In vivo imaging
  • Luminescence
  • Recombinant Nipah virus
  • Reporter genes
  • Rodent animal model

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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