In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus.

Allison Poussard, Michael Patterson, Katherine Taylor, Alexey Seregin, Jeanon Smith, Jennifer Smith, Milagros Salazar, Slobodan Paessler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Modern advancements in imaging technology encourage further development and refinement in the way viral research is accomplished. Initially proposed by Russel and Burch in Hume's 3Rs (replacement, reduction, refinement), the utilization of animal models in scientific research is under constant pressure to identify new methodologies to reduce animal usage while improving scientific accuracy and speed. A major challenge to Hume's principals however, is how to ensure the studies are statistically accurate while reducing animal disease morbidity and overall numbers. Vaccine efficacy studies currently require a large number of animals in order to be considered statistically significant and often result in high morbidity and mortality endpoints for identification of immune protection. We utilized in vivo imaging systems (IVIS) in conjunction with a firefly bioluminescent enzyme to progressively track the invasion of the central nervous system (CNS) by an encephalitic virus in a murine model. Typically, the disease progresses relatively slowly, however virus replication is rapid, especially within the CNS, and can lead to an often, lethal outcome. Following intranasal infection of the mice with TC83-Luc, an attenuated Venezuelan equine encephalitis virus strain modified to expresses a luciferase gene; we are able to visualize virus replication within the brain at least three days before the development of clinical disease symptoms. Utilizing CNS invasion as a key encephalitic disease development endpoint we are able to quickly identify therapeutic and vaccine protection against TC83-Luc infection before clinical symptoms develop. With IVIS technology we are able to demonstrate the rapid and accurate testing of drug therapeutics and vaccines while reducing animal numbers and morbidity.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number70
StatePublished - 2012
Externally publishedYes

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Viruses
Imaging systems
Animals
Vaccines
Central Nervous System
Virus Replication
Morbidity
Neurology
Fireflies
Venezuelan Equine Encephalitis Viruses
Technology
Animal Diseases
Infection
Luciferases
Research
Animal Models
Pressure
Mortality
Brain
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Poussard, A., Patterson, M., Taylor, K., Seregin, A., Smith, J., Smith, J., ... Paessler, S. (2012). In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus. Journal of visualized experiments : JoVE, (70).

In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus. / Poussard, Allison; Patterson, Michael; Taylor, Katherine; Seregin, Alexey; Smith, Jeanon; Smith, Jennifer; Salazar, Milagros; Paessler, Slobodan.

In: Journal of visualized experiments : JoVE, No. 70, 2012.

Research output: Contribution to journalArticle

Poussard, A, Patterson, M, Taylor, K, Seregin, A, Smith, J, Smith, J, Salazar, M & Paessler, S 2012, 'In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus.', Journal of visualized experiments : JoVE, no. 70.
Poussard A, Patterson M, Taylor K, Seregin A, Smith J, Smith J et al. In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus. Journal of visualized experiments : JoVE. 2012;(70).
Poussard, Allison ; Patterson, Michael ; Taylor, Katherine ; Seregin, Alexey ; Smith, Jeanon ; Smith, Jennifer ; Salazar, Milagros ; Paessler, Slobodan. / In vivo imaging systems (IVIS) detection of a neuro-invasive encephalitic virus. In: Journal of visualized experiments : JoVE. 2012 ; No. 70.
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