In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy

Kert Edward, Suimin Qiu, Vicente Resto, Susan McCammon, Gracie Vargas

Research output: Contribution to journalArticle

13 Scopus citations


Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.

Original languageEnglish (US)
Pages (from-to)1579-1593
Number of pages15
JournalBiomedical Optics Express
Issue number7
StatePublished - Jul 1 2012


ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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