Abstract
Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1579-1593 |
| Number of pages | 15 |
| Journal | Biomedical Optics Express |
| Volume | 3 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2012 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Biotechnology
Cite this
In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy. / Edward, Kert; Qiu, Suimin; Resto, Vicente; McCammon, Susan; Vargas, Gracie.
In: Biomedical Optics Express, Vol. 3, No. 7, 01.07.2012, p. 1579-1593.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy
AU - Edward, Kert
AU - Qiu, Suimin
AU - Resto, Vicente
AU - McCammon, Susan
AU - Vargas, Gracie
PY - 2012/7/1
Y1 - 2012/7/1
N2 - Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.
AB - Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.
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UR - http://www.scopus.com/inward/citedby.url?scp=84863903420&partnerID=8YFLogxK
U2 - 10.1364/BOE.3.001579
DO - 10.1364/BOE.3.001579
M3 - Article
VL - 3
SP - 1579
EP - 1593
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 7
ER -