TY - JOUR
T1 - In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy
AU - Edward, Kert
AU - Qiu, Suimin
AU - Resto, Vicente
AU - McCammon, Susan
AU - Vargas, Gracie
PY - 2012/7/1
Y1 - 2012/7/1
N2 - Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.
AB - Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.
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U2 - 10.1364/BOE.3.001579
DO - 10.1364/BOE.3.001579
M3 - Article
C2 - 22808430
AN - SCOPUS:84863903420
VL - 3
SP - 1579
EP - 1593
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 7
ER -