In vivo magnetic resonance imaging of vascularization in islet transplantation

Eba Hathout, Lawrence Sowers, Rong Wang, Annie Tan, John Mace, Ricardo Peverini, Richard Chinnock, Andre Obenaus

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

To evaluate changes in neovascularization of transplanted islets in vivo, dynamic contrast (gadolinium) enhanced magnetic resonance imaging (MRI) was used. Both iron (Feridex)-labeled and unlabeled syngeneic murine subcapsular islet grafts were studied. Differences in dynamic contrast enhancement of islet grafts were quantified after gadolinium injection at post-transplant days 3 and 14. Normalized contrast concentrations at day 14 in transplanted islets were increased relative with that on day 3. Time to peak contrast enhancement was faster by 12 min at day 14 compared to day 3 islets (while kidney and muscle peak times remained the same). Areas under the curve for contrast concentration versus time plots were larger in 14-day relative to 3-day islet grafts. In conclusion, noninvasive assessment of neovascularization is achievable. In vivo dynamic contrast-enhanced MRI can be used to detect and quantify changes in vascularization following islet transplantation. This technique may be useful in developing pro-angiogenic strategies to improve the transplantation outcome in experimental and clinical settings.

Original languageEnglish (US)
Pages (from-to)1059-1065
Number of pages7
JournalTransplant International
Volume20
Issue number12
DOIs
StatePublished - Dec 1 2007

    Fingerprint

Keywords

  • Imaging
  • Islet transplant
  • Islet vascularization

ASJC Scopus subject areas

  • Transplantation

Cite this

Hathout, E., Sowers, L., Wang, R., Tan, A., Mace, J., Peverini, R., Chinnock, R., & Obenaus, A. (2007). In vivo magnetic resonance imaging of vascularization in islet transplantation. Transplant International, 20(12), 1059-1065. https://doi.org/10.1111/j.1432-2277.2007.00550.x