TY - JOUR
T1 - In vivo model for the acute, latent and reactivated phases of cytomegalovirus infection.
AU - Gönczöl, E.
AU - Danczig, E.
AU - Boldogh, I.
AU - Tóth, T.
AU - Váczi, L.
N1 - Funding Information:
RFB and JPK conceived the project and acquired the grant funds. AWT, DMB, QB, NTS, PLR, and RFB designed the protocol and conducted the analysis. TM and SN directed the data management. AWT, DMB, and QB drafted and revised the manuscript. DO, KLK, SEA, IM, RC, VLB, VA, MDT, NTS, AK, TM, SN, NA, ACS, JAGS, RK, AJ, BATB, ESG, JO, SRZ, SOS, FI, AR, PLR and JPK participated in protocol development and coordinated the clinical and diagnostic data collection. SFD reviewed the manuscript and provided non-binding input. RFB and SAM had final responsibility for the decision to submit for publication. All authors reviewed the draft and approved the decision to submit for publication.
Funding Information:
SAM reports grants from the Bill & Melinda Gates Foundation, Pfizer, GlaxoSmithKline, Minervax, and Sanofi, and honoraria from the Gates Foundation, outside the submitted work. All other authors declare no competing interests.
PY - 1985
Y1 - 1985
N2 - Tissue samples from the salivary gland, lung spleen, liver and kidney of Balb/c mice infected with murine cytomegalovirus (MCMV) contained infective MCMV and virus-specific antigens for 6-7 weeks following the infection. After the 8th week no infective virus could be detected in any organ and antigens were found only in the spleen and the mesangial cells of the renal glomerules. As a result of cyclophosphamide treatment applied in the 22nd-24th weeks, the latent viral infection was reactivated in nearly all animals, infective virus production started again in the organs, and lasted for about 3 weeks. During the subsequent latent period the virus was again reactivated by repeated cyclophosphamide treatment. The experimental alternation of the latent and reactivated phases of viral infection renders the model suitable for study of the mechanism and consequences of viral latency and reactivation in vivo.
AB - Tissue samples from the salivary gland, lung spleen, liver and kidney of Balb/c mice infected with murine cytomegalovirus (MCMV) contained infective MCMV and virus-specific antigens for 6-7 weeks following the infection. After the 8th week no infective virus could be detected in any organ and antigens were found only in the spleen and the mesangial cells of the renal glomerules. As a result of cyclophosphamide treatment applied in the 22nd-24th weeks, the latent viral infection was reactivated in nearly all animals, infective virus production started again in the organs, and lasted for about 3 weeks. During the subsequent latent period the virus was again reactivated by repeated cyclophosphamide treatment. The experimental alternation of the latent and reactivated phases of viral infection renders the model suitable for study of the mechanism and consequences of viral latency and reactivation in vivo.
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M3 - Article
C2 - 2994350
AN - SCOPUS:0021780666
SN - 1217-8950
VL - 32
SP - 39
EP - 47
JO - Acta Microbiologica et Immunologica Hungarica
JF - Acta Microbiologica et Immunologica Hungarica
IS - 1
ER -