TY - JOUR
T1 - In Vivo Model of Bone Regeneration with Aspirin Cross-linked O-carboxymethyl Chitosan Hydrogel Loaded with Adipose Derived-Stem Cells
AU - Hosseinzadeh, Simzar
AU - Shahghasempour, Lida
AU - Abbaszadeh, Hojjat Allah
AU - Soleimani, Masoud
AU - Abdollahifar, Mohammad Amin
AU - Rasouli, Mehdi
AU - Khani, Mohammad Mehdi
AU - Fattahi, Roya
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.
AB - Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.
KW - 3D Hydrogel
KW - Aspirin
KW - Bone Regeneration
KW - Cross-linker
KW - Human Adipocyte Mesenchymal Stem Cell
KW - In Vivo
KW - O- Carboxymethyl Chitosan
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U2 - 10.1007/s10924-025-03571-y
DO - 10.1007/s10924-025-03571-y
M3 - Article
AN - SCOPUS:105003586017
SN - 1566-2543
JO - Journal of Polymers and the Environment
JF - Journal of Polymers and the Environment
ER -