In vivo mucosal tissue imaging using fiber-based two-photon approach

Xiao Xudong, Shilagard Tuya, Gracie Vargas

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Light delivery by fiber can directly excite the desired region. In recent years a number of methods have been proposed for fiber-based multiphoton microscopy. Of particular interest is the type of fiber used for both excitation with femtosecond pulses and method of collection of emitted fluorescence. In our efforts towards the development of a compact multiphoton microendoscope, we have conducted a study comparing the performance of three types of fibers (single mode fiber, hollow core photonic crystal fiber, and double clad fiber) in order to provide an objective comparison between various excitation approaches. In this study, we have demonstrated the ability of multiphoton microscopy and second harmonic generation microscopy for high resolution intensity and life time imaging of the oral mucosa and submucosa in vivo based on the use of double clad photonic crystal fiber.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6442
DOIs
StatePublished - 2007
EventMultiphoton Microscopy in the Biomedical Sciences VII - San Jose, CA, United States
Duration: Jan 21 2007Jan 23 2007

Other

OtherMultiphoton Microscopy in the Biomedical Sciences VII
CountryUnited States
CitySan Jose, CA
Period1/21/071/23/07

Fingerprint

Photons
Imaging techniques
Fibers
Microscopic examination
Photonic crystal fibers
Single mode fibers
Harmonic generation
Ultrashort pulses
Fluorescence
Mucous Membrane

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Xudong, X., Tuya, S., & Vargas, G. (2007). In vivo mucosal tissue imaging using fiber-based two-photon approach. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6442). [644220] https://doi.org/10.1117/12.708017

In vivo mucosal tissue imaging using fiber-based two-photon approach. / Xudong, Xiao; Tuya, Shilagard; Vargas, Gracie.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6442 2007. 644220.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xudong, X, Tuya, S & Vargas, G 2007, In vivo mucosal tissue imaging using fiber-based two-photon approach. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6442, 644220, Multiphoton Microscopy in the Biomedical Sciences VII, San Jose, CA, United States, 1/21/07. https://doi.org/10.1117/12.708017
Xudong X, Tuya S, Vargas G. In vivo mucosal tissue imaging using fiber-based two-photon approach. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6442. 2007. 644220 https://doi.org/10.1117/12.708017
Xudong, Xiao ; Tuya, Shilagard ; Vargas, Gracie. / In vivo mucosal tissue imaging using fiber-based two-photon approach. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6442 2007.
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