In vivo multimodal nonlinear optical imaging of mucosal tissue

Ju Sun, Tuya Shilagard, Brent Bell, Massoud Motamedi, Gracie Vargas

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


We present a multimodal nonlinear imaging approach to elucidate microstructures and spectroscopic features of oral mucosa and submucosa in vivo. The hamster buccal pouch was imaged using 3-D high resolution multiphoton and second harmonic generation microscopy. The multimodal imaging approach enables colocalization and differentiation of prominent known spectroscopic and structural features such as keratin, epithelial cells, and submucosal collagen at various depths in tissue. Visualization of cellular morphology and epithelial thickness are in excellent agreement with histological observations. These results suggest that multimodal nonlinear optical microscopy can be an effective tool for studying the physiology and pathology of mucosal tissue.

Original languageEnglish (US)
Pages (from-to)2478-2486
Number of pages9
JournalOptics Express
Issue number11
StatePublished - May 31 2004

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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