In vivo multiphoton and second harmonic generation microscopy of epithelial carcinogenesis

Gracie Vargas, Tuya Shilagard, Ju Sun, Massoud Motamedi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations


Multiphoton microscopy and second harmonic generation microscopy were used to image epithelial changes in a hamster model for oral malignant transformation. In vivo imaging was performed to characterize morphometric alterations in normal and precancerous regions. Morphometric measurements such as cell nucleus area and epithelial thicknesses obtained from MPM-SHGM were in excellent agreement with histology obtained after in vivo imaging. MPM-SHGM was highly sensitive to spectroscopic and architectural alterations throughout carcinogenesis, showing statistically significant changes in morphology. MPM revealed hyperkeratosis, nuclear enlargement/crowding in dysplasia, and immune cell infiltration. SHGM revealed alterations in submucosal architecture, with a decrease in SHG density evident during early stages of precancer. By combining MPM with SHGM, the basement membrane could be identified in normal, hyperplasia, and dysplasia samples and in some cases of early invasion. The combined technique of MPM-SHGM has the potential to serve as an adjunct to biopsy for assessing precancerous changes and will be investigated further for that purpose. Additionally, the method can provide spatiotemporal assessment of early neoplastic changes in order to elucidate the stages of transformation in vivo and could be used to assess therapeutic efficacy of agents being tested for the treatment of epithelial precancers/cancer.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2006
EventOptical Biopsy VI - San Jose, CA, United States
Duration: Jan 24 2006Jan 24 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherOptical Biopsy VI
Country/TerritoryUnited States
CitySan Jose, CA


  • Multiphoton microscopy
  • Oral precancer
  • Second harmonic generation microscopy
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


Dive into the research topics of 'In vivo multiphoton and second harmonic generation microscopy of epithelial carcinogenesis'. Together they form a unique fingerprint.

Cite this