TY - GEN
T1 - In vivo multiphoton and second harmonic generation microscopy of epithelial carcinogenesis
AU - Vargas, Gracie
AU - Shilagard, Tuya
AU - Sun, Ju
AU - Motamedi, Massoud
PY - 2006
Y1 - 2006
N2 - Multiphoton microscopy and second harmonic generation microscopy were used to image epithelial changes in a hamster model for oral malignant transformation. In vivo imaging was performed to characterize morphometric alterations in normal and precancerous regions. Morphometric measurements such as cell nucleus area and epithelial thicknesses obtained from MPM-SHGM were in excellent agreement with histology obtained after in vivo imaging. MPM-SHGM was highly sensitive to spectroscopic and architectural alterations throughout carcinogenesis, showing statistically significant changes in morphology. MPM revealed hyperkeratosis, nuclear enlargement/crowding in dysplasia, and immune cell infiltration. SHGM revealed alterations in submucosal architecture, with a decrease in SHG density evident during early stages of precancer. By combining MPM with SHGM, the basement membrane could be identified in normal, hyperplasia, and dysplasia samples and in some cases of early invasion. The combined technique of MPM-SHGM has the potential to serve as an adjunct to biopsy for assessing precancerous changes and will be investigated further for that purpose. Additionally, the method can provide spatiotemporal assessment of early neoplastic changes in order to elucidate the stages of transformation in vivo and could be used to assess therapeutic efficacy of agents being tested for the treatment of epithelial precancers/cancer.
AB - Multiphoton microscopy and second harmonic generation microscopy were used to image epithelial changes in a hamster model for oral malignant transformation. In vivo imaging was performed to characterize morphometric alterations in normal and precancerous regions. Morphometric measurements such as cell nucleus area and epithelial thicknesses obtained from MPM-SHGM were in excellent agreement with histology obtained after in vivo imaging. MPM-SHGM was highly sensitive to spectroscopic and architectural alterations throughout carcinogenesis, showing statistically significant changes in morphology. MPM revealed hyperkeratosis, nuclear enlargement/crowding in dysplasia, and immune cell infiltration. SHGM revealed alterations in submucosal architecture, with a decrease in SHG density evident during early stages of precancer. By combining MPM with SHGM, the basement membrane could be identified in normal, hyperplasia, and dysplasia samples and in some cases of early invasion. The combined technique of MPM-SHGM has the potential to serve as an adjunct to biopsy for assessing precancerous changes and will be investigated further for that purpose. Additionally, the method can provide spatiotemporal assessment of early neoplastic changes in order to elucidate the stages of transformation in vivo and could be used to assess therapeutic efficacy of agents being tested for the treatment of epithelial precancers/cancer.
KW - Multiphoton microscopy
KW - Oral precancer
KW - Second harmonic generation microscopy
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=33646196198&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646196198&partnerID=8YFLogxK
U2 - 10.1117/12.646564
DO - 10.1117/12.646564
M3 - Conference contribution
AN - SCOPUS:33646196198
SN - 0819461334
SN - 9780819461339
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
T2 - Optical Biopsy VI
Y2 - 24 January 2006 through 24 January 2006
ER -