@article{ea79b341047648f792cdee4b8e03e392,
title = "In vivo observation of structural changes in neocortical catecholaminergic projections in response to drugs of abuse",
abstract = "Catecholaminergic (dopamine and norepinephrine) projections to the cortex play an important role in cognitive functions and dysfunctions including learning, addiction, and mental disorders. While dynamics of glutamatergic synapses have been well studied in such contexts, little is known regarding catecholaminergic projections, owing to lack of robust methods. Here we report a system to monitor catecholaminergic projections in vivo over the timeframes that such events occur. Green fluorescent protein (GFP) expression driven by tyrosine hydroxylase promoter in a transgenic mouse line enabled us to perform two-photon imaging of cortical catecholaminergic projections through a cranial window. Repetitive imaging of the same axons over 24 h revealed the highly dynamic nature of catecholaminergic boutons. Surprisingly, administration of single high dose methamphetamine (MAP) induced a transient increase in bouton volumes. This new method opens avenues for longitudinal in vivo evaluation of structural changes at single release sites of catecholamines in association with physiology and pathology of cortical functions.",
keywords = "Axon, Catecholamine, In vivo imaging, Methamphetamine, Neocortex, Transgenic mouse",
author = "Morimoto, {Mai M.} and Shinji Tanaka and Shunsuke Mizutani and Shinji Urata and Kazuto Kobayashi and Shigeo Okabe",
note = "Funding Information: Received March 2, 2017; accepted January 4, 2018; First published January 22, 2018. The authors declare no competing financial interests. Author contribution: M.M.M., K.K., and S.O. designed research; M.M.M., S.T., S.M., and S.U. performed research; M.M.M. analyzed data; M.M.M. and S.O. wrote the paper. This work was supported by Grants-in-Aid for Scientific Research 17H01387, 26250014, and 25117006 (to S.O.) and the Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency Grant JPMJCR14W2 (to S.O.), Uehara Memorial Foundation (S.O.), and University of Tokyo Center for Integrative Science of Human Behavior. ‡Current address: Department of Experimental Psychology, University College London, 26 Bedford Way, WC1H 0AP, London, UK. Acknowledgements: We thank Dr. Satoru Kondo for assistance with two-photon microscopy, Dr. Susumu Jitsuki for conducting the microdialysis experiments, and all members of the Okabe lab for discussions and comments. Correspondence should be addressed to Shigeo Okabe at the above address, E-mail: okabe@m.u-tokyo.ac.jp. DOI:http://dx.doi.org/10.1523/ENEURO.0071-17.2018 Copyright {\textcopyright} 2018 Morimoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Funding Information: This work was supported by Grants-in-Aid for Scientific Research 17H01387, 26250014, and 25117006 (to S.O.) and the Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency Grant JPMJCR14W2 (to S.O.), Uehara Memorial Foundation (S.O.), and University of Tokyo Center for Integrative Science of Human Behavior. Publisher Copyright: {\textcopyright} 2018 Morimoto et al.",
year = "2018",
month = jan,
day = "1",
doi = "10.1523/ENEURO.0071-17.2018",
language = "English (US)",
volume = "5",
journal = "eNeuro",
issn = "2373-2822",
publisher = "Society for Neuroscience",
number = "1",
}