In Vivo Ovine Flap Model to Evaluate Surgical Infection and Tissue Necrosis

Linda Phillips, Roberta Mann, John P. Heggers, Hugo A. Linares, Martin C. Robson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although the inciting causes and the end results of soft tissue infection are well described, we have not achieved a quantitative methodistic description of the sequence of events in between. The following study presents an isolated in vivo model which will allow specific manipulations and quantifications of the events of integumentary infection. Pedicle flaps were raised on the buttock of the adult range ewe and it was multiply inoculated with Staphylococcus aureus, and followed for 96 hr. This pedicle contains not only a well-defined artery, vein, and nerve, but also a well-developed efferent lymphatic vessel. In addition, the distal portion of the flap is primarily supplied by a musculocutaneous arterial perforator, making the distal flap a watershed area. Anatomic, radiologic, and vital stain injection studies confirmed these results. The sheep model was found to be superior to the pig model both in terms of its anatomy (the addition of an efferent lymphatic vessel) as well as the disposition of the sheep which was more compliant than that of the pig. We believe that this flap will allow multiple manipulations and provide an in vivo isolated system to study the pathobiology of soft tissue infection.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalJournal of Surgical Research
Volume56
Issue number1
DOIs
StatePublished - Jan 1994

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Soft Tissue Infections
Lymphatic Vessels
Sheep
Necrosis
Swine
Buttocks
Infection
Staphylococcus aureus
Veins
Anatomy
Coloring Agents
Arteries
Injections

ASJC Scopus subject areas

  • Surgery

Cite this

In Vivo Ovine Flap Model to Evaluate Surgical Infection and Tissue Necrosis. / Phillips, Linda; Mann, Roberta; Heggers, John P.; Linares, Hugo A.; Robson, Martin C.

In: Journal of Surgical Research, Vol. 56, No. 1, 01.1994, p. 1-4.

Research output: Contribution to journalArticle

Phillips, Linda ; Mann, Roberta ; Heggers, John P. ; Linares, Hugo A. ; Robson, Martin C. / In Vivo Ovine Flap Model to Evaluate Surgical Infection and Tissue Necrosis. In: Journal of Surgical Research. 1994 ; Vol. 56, No. 1. pp. 1-4.
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