In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis

Danny O. Jacobs, John Maris, Robert Fried, R. Gregg Settle, Rolando R. Rolandelli, Mark J. Koruda, Briton Chance, John L. Rombeau

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

High-energy phosphate metabolism in skeletal muscle is altered during sepsis, although the chronology of events is uncertain. Phosphorus 31 magnetic resonance spectroscopy was used to measure changes in muscle energy stores of the left hind limb musculature of adult male rats during sepsis. Following control scans, cecal ligation and puncture were performed and scanning was repeated 6, 24, and 48 hours after surgery. The ratios of phosphocreatine (PCr) to inorganic phosphate (Pi), a measure of energy stores, and adenosine triphosphate (ATP) to Pi ratio, a measure of the energy available for immediate use, were determined from peak heights. Intracellular pH was calculated using the distance between Pi and PCr peaks. In surviving animals, a 40% decrease in PCr/Pi ratio (±SEM) was observed by 24 hours (22.3±3.0 at time 0 vs 13.3±2.8 at 24 hours), whereas energy availability (β-ATP/Pi) was statistically unchanged (18.2 ±2.2 at time 0 vs 15.2± 1.2 at 48 hours). Intracellular pH did not change. Both PCr/Pi and ATP/Pi ratios were inversely correlated with time. In this model of documented peritonitis, skeletal muscle energy metabolism is rapidly altered following severe infection, and these changes can be detected using 31P magnetic resonance spectroscopy.

Original languageEnglish (US)
Pages (from-to)1425-1428
Number of pages4
JournalArchives of Surgery
Volume123
Issue number11
DOIs
StatePublished - Nov 1988

ASJC Scopus subject areas

  • Surgery

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    Jacobs, D. O., Maris, J., Fried, R., Settle, R. G., Rolandelli, R. R., Koruda, M. J., Chance, B., & Rombeau, J. L. (1988). In Vivo Phosphorus 31 Magnetic Resonance Spectroscopy of Rat Hind Limb Skeletal Muscle During Sepsis. Archives of Surgery, 123(11), 1425-1428. https://doi.org/10.1001/archsurg.1988.01400350139022