@article{1416f675595948998bad569d11941b1b,
title = "In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus",
abstract = "Six seronegative owl monkeys were intravenously inoculated with an antigenic variant (SI8) of hepatitis A virus that is highly adapted to growth in cell culture and resists neutralization by monoclonal antibodies due to replacement of aspartic acid 70 of capsid protein VP3 with histidine. Each developed hepatitis 2233 days after inoculation. Virus in feces, serum, and liver was quantified by radioimmunofocus assay. Viremia developed 711 days after inoculation, in parallel with fecal shedding of virus, and persisted for a mean of 20.5 days. Although the antigenic variant was recovered from feces or liver of three animals, virus in liver at the time of enzyme elevations was predominantly wild-type antigenic phenotype. Virus was not recoveredfrom liver 96 days after challenge. These studies further define virologic events in hepatitis A and show that in vivo replication of an antigenic variant was restricted compared with that of wildtype virus.",
author = "Lemon, {Stanley M.} and Binn, {Leonard N.} and Ruth Marchwicki and Murphy, {Paula C.} and Ping, {Li Hua} and Jansen, {Robert W.} and Asher, {Ludmilla V.S.} and Stapleton, {Jack T.} and Taylor, {Dewayne G.} and LeDuc, {James W.}",
note = "Funding Information: Received 17 April 1989; revised 10 July 1989. Presented in part at the International Symposium on Viral Hepatitis and Liver Disease, 26-28 May 1987, London. Guidelines of the Committee on the Care and Use of Laboratory Animals of the National Research Council were followed; facilities were fully accredited by the American Association for Accreditation of Laboratory Animal Care. The views of the authors do not purport to reflect the positions of the Department of the Army or the Department of Defense. Supported in part by contract DAMDI7-85C-5272 from the US Army Medical Research and Development Command and by grant AI-22279 from the National Institute of Allergy and Infectious Diseases. Reprints and correspondence: Dr. S. M. Lemon, Division of Infectious Diseases, 547 Burnett-Womack CB 7030, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7030. * Present address: Division of Infectious Diseases, University of Iowa, Iowa City.",
year = "1990",
month = jan,
doi = "10.1093/infdis/161.1.7",
language = "English (US)",
volume = "161",
pages = "7--13",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "1",
}