In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus

Stanley M. Lemon, Leonard N. Binn, Ruth Marchwicki, Paula C. Murphy, Li Hua Ping, Robert W. Jansen, Ludmilla V S Asher, Jack T. Stapleton, Dewayne G. Taylor, James LeDuc

Research output: Contribution to journalArticle

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Abstract

Six seronegative owl monkeys were intravenously inoculated with an antigenic variant (S18) of hepatitis A virus that is highly adapted to growth in cell culture and resists neutralization by monoclonal antibodies due to replacement of aspartic acid 70 of capsid protein VP3 with histidine. Each developed hepatitis 22-33 days after inoculation. Virus in feces, serum, and liver was quantified by radioimmunofocus assay. Viremia developed 7-11 days after inoculation, in parallel with fecal shedding of virus, and persisted for a mean of 20.5 days. Although the antigenic variant was recovered from feces or liver of three animals, virus in liver at the time of enzyme elevations was predominantly wild-type antigenic phenotype. Virus was not recovered from liver 96 days after challenge. These studies further define virologic events in hepatitis A and show that in vivo replication of an antigenic variant was restricted compared with that of wildtype virus.

Original languageEnglish (US)
Pages (from-to)7-13
Number of pages7
JournalJournal of Infectious Diseases
Volume161
Issue number1
StatePublished - Jan 1990
Externally publishedYes

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Hepatitis A virus
Viruses
Liver
Feces
Aotidae
Virus Shedding
Hepatitis A
Viremia
Capsid Proteins
Histidine
Aspartic Acid
Hepatitis
Cell Culture Techniques
Monoclonal Antibodies
Phenotype
Enzymes
Growth
Serum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Lemon, S. M., Binn, L. N., Marchwicki, R., Murphy, P. C., Ping, L. H., Jansen, R. W., ... LeDuc, J. (1990). In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus. Journal of Infectious Diseases, 161(1), 7-13.

In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus. / Lemon, Stanley M.; Binn, Leonard N.; Marchwicki, Ruth; Murphy, Paula C.; Ping, Li Hua; Jansen, Robert W.; Asher, Ludmilla V S; Stapleton, Jack T.; Taylor, Dewayne G.; LeDuc, James.

In: Journal of Infectious Diseases, Vol. 161, No. 1, 01.1990, p. 7-13.

Research output: Contribution to journalArticle

Lemon, SM, Binn, LN, Marchwicki, R, Murphy, PC, Ping, LH, Jansen, RW, Asher, LVS, Stapleton, JT, Taylor, DG & LeDuc, J 1990, 'In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus', Journal of Infectious Diseases, vol. 161, no. 1, pp. 7-13.
Lemon, Stanley M. ; Binn, Leonard N. ; Marchwicki, Ruth ; Murphy, Paula C. ; Ping, Li Hua ; Jansen, Robert W. ; Asher, Ludmilla V S ; Stapleton, Jack T. ; Taylor, Dewayne G. ; LeDuc, James. / In vivo replication and reversion to wild type of a neutralization-resistant antigenic variant of hepatitis A virus. In: Journal of Infectious Diseases. 1990 ; Vol. 161, No. 1. pp. 7-13.
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