In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

Bernard Choi; Boris Majaron; Gracie Vargas; Byungjo Jung; Oliver Stumpp; Nicole M. Kang; Kristen M. Kelly; Ashley J. Welch; J. Stuart Nelson

doi:10.1117/12.484140

In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

Bernard Choi, Boris Majaron, Gracie Vargas, Byungjo Jung, Oliver Stumpp, Nicole M. Kang, Kristen M. Kelly, Ashley J. Welch, J. Stuart Nelson

Research output: Contribution to journal › Conference article › peer-review

6 Scopus citations

Abstract

Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.

Original language	English (US)
Pages (from-to)	350-361
Number of pages	12
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5047
DOIs	https://doi.org/10.1117/12.484140
State	Published - 2003
Externally published	Yes
Event	PROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II - San Diego, CA, United States Duration: Mar 3 2003 → Mar 5 2003

Keywords

Inverse problem
Microvasculature
Pulsed photothermal radiometry
Tomography
Vascular lesion

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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title = "In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels",

abstract = "Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.",

keywords = "Inverse problem, Microvasculature, Pulsed photothermal radiometry, Tomography, Vascular lesion",

author = "Bernard Choi and Boris Majaron and Gracie Vargas and Byungjo Jung and Oliver Stumpp and Kang, {Nicole M.} and Kelly, {Kristen M.} and Welch, {Ashley J.} and Nelson, {J. Stuart}",

year = "2003",

doi = "10.1117/12.484140",

language = "English (US)",

volume = "5047",

pages = "350--361",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "PROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II ; Conference date: 03-03-2003 Through 05-03-2003",

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TY - JOUR

T1 - In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

AU - Choi, Bernard

AU - Majaron, Boris

AU - Vargas, Gracie

AU - Jung, Byungjo

AU - Stumpp, Oliver

AU - Kang, Nicole M.

AU - Kelly, Kristen M.

AU - Welch, Ashley J.

AU - Nelson, J. Stuart

PY - 2003

Y1 - 2003

N2 - Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.

AB - Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.

KW - Inverse problem

KW - Microvasculature

KW - Pulsed photothermal radiometry

KW - Tomography

KW - Vascular lesion

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DO - 10.1117/12.484140

M3 - Conference article

AN - SCOPUS:0242693958

SN - 0277-786X

VL - 5047

SP - 350

EP - 361

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - PROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II

Y2 - 3 March 2003 through 5 March 2003

ER -

In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

Abstract

Keywords

ASJC Scopus subject areas

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