In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

Bernard Choi, Boris Majaron, Gracie Vargas, Byungjo Jung, Oliver Stumpp, Nicole M. Kang, Kristen M. Kelly, Ashley J. Welch, J. Stuart Nelson

Research output: Contribution to journalConference article

6 Scopus citations

Abstract

Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.

Original languageEnglish (US)
Pages (from-to)350-361
Number of pages12
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5047
DOIs
StatePublished - Nov 27 2003
EventPROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II - San Diego, CA, United States
Duration: Mar 3 2003Mar 5 2003

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Keywords

  • Inverse problem
  • Microvasculature
  • Pulsed photothermal radiometry
  • Tomography
  • Vascular lesion

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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