In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels

Bernard Choi, Boris Majaron, Gracie Vargas, Byungjo Jung, Oliver Stumpp, Nicole M. Kang, Kristen M. Kelly, Ashley J. Welch, J. Stuart Nelson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Previous studies suggest that optimal port wine stain (PWS) laser treatment parameters require knowledge of skin characteristics such as blood vessel size, depth, and distribution. Effective and rapid imaging modalities are not widely available. In the present study, photothermal tomography (PTT) images of an in vivo hamster window model and human PWS skin were obtained and analyzed. Subtherapeutic laser light pulses at 585 and 600 nm were applied to skin surface and image sequences acquired with an infrared camera. A nonnegatively constrained conjugate gradient algorithm was used to reconstruct a PTT image of the initial temperature distribution immediately following pulsed laser irradiation. Vessel dimensions determined from PTT images of hamster window model skin compared well with those measured directly using video microscopy. PTT images of human PWS skin contained vessels with estimated diameters of 200-250 μm over a 250-320 μm depth range. Use of dual wavelength excitation (DWE) analysis allowed for imaging of shallow vessels.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsT. Kundu
Pages350-361
Number of pages12
Volume5047
DOIs
StatePublished - 2003
Externally publishedYes
EventPROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II - San Diego, CA, United States
Duration: Mar 3 2003Mar 5 2003

Other

OtherPROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II
CountryUnited States
CitySan Diego, CA
Period3/3/033/5/03

Fingerprint

blood vessels
Blood vessels
Tomography
Skin
wines
tomography
Wine
Imaging techniques
vessels
hamsters
Lasers
Laser beam effects
Pulsed lasers
lasers
Laser pulses
pulsed lasers
Microscopic examination
Temperature distribution
temperature distribution
Cameras

Keywords

  • Inverse problem
  • Microvasculature
  • Pulsed photothermal radiometry
  • Tomography
  • Vascular lesion

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Choi, B., Majaron, B., Vargas, G., Jung, B., Stumpp, O., Kang, N. M., ... Nelson, J. S. (2003). In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels. In T. Kundu (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5047, pp. 350-361) https://doi.org/10.1117/12.484140

In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels. / Choi, Bernard; Majaron, Boris; Vargas, Gracie; Jung, Byungjo; Stumpp, Oliver; Kang, Nicole M.; Kelly, Kristen M.; Welch, Ashley J.; Nelson, J. Stuart.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / T. Kundu. Vol. 5047 2003. p. 350-361.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Choi, B, Majaron, B, Vargas, G, Jung, B, Stumpp, O, Kang, NM, Kelly, KM, Welch, AJ & Nelson, JS 2003, In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels. in T Kundu (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5047, pp. 350-361, PROCEEDINGS OF SPIE SPIE - The International Society for Optical Engineering: Smart Nondestructive Evaluation and Health Monitoring of Structural and Biological Systems II, San Diego, CA, United States, 3/3/03. https://doi.org/10.1117/12.484140
Choi B, Majaron B, Vargas G, Jung B, Stumpp O, Kang NM et al. In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels. In Kundu T, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5047. 2003. p. 350-361 https://doi.org/10.1117/12.484140
Choi, Bernard ; Majaron, Boris ; Vargas, Gracie ; Jung, Byungjo ; Stumpp, Oliver ; Kang, Nicole M. ; Kelly, Kristen M. ; Welch, Ashley J. ; Nelson, J. Stuart. / In Vivo Results Using Photothermal Tomography for Imaging Cutaneous Blood Vessels. Proceedings of SPIE - The International Society for Optical Engineering. editor / T. Kundu. Vol. 5047 2003. pp. 350-361
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