In vivo selection of H1N2 influenza virus reassortants in the Ferret model

Matthew Angel, J. Brian Kimble, Lindomar Pena, Hongquan Wan, Daniel R. Perez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although the ferret model has been extensively used to study pathogenesis and transmission of influenza viruses, little has been done to determine whether ferrets are a good surrogate animal model to study influenza virus reassortment. It has been previously shown that the pandemic 2009 H1N1 (H1N1pdm) virus was able to transmit efficiently in ferrets. In coinfection studies with either seasonal H1N1 or H3N2 strains (H1N1s or H3N2s, respectively), the H1N1pdm virus was able to outcompete these strains and become the dominant transmissible virus. However, lack of reassortment could have been the result of differences in the cell or tissue tropism of these viruses in the ferret. To address this issue, we performed coinfection studies with recombinant influenza viruses carrying the surface genes of a seasonal H3N2 strain in the background of an H1N1pdm strain and vice versa. After serial passages in ferrets, a dominant H1N2 virus population was obtained with a constellation of gene segments, most of which, except for the neuraminidase (NA) and PB1 segments, were from the H1N1pdm strain. Our studies suggest that ferrets recapitulate influenza virus reassortment events. The H1N2 virus generated through this process resembles similar viruses that are emerging in nature, particularly in pigs.

Original languageEnglish (US)
Pages (from-to)3277-3283
Number of pages7
JournalJournal of Virology
Volume87
Issue number6
DOIs
StatePublished - Mar 2013
Externally publishedYes

Fingerprint

H1N2 Subtype Influenza A Virus
Ferrets
ferrets
Orthomyxoviridae
Influenza A virus
Viruses
viruses
Coinfection
mixed infection
tissue tropism
Serial Passage
H1N1 Subtype Influenza A Virus
Tropism
sialidase
Neuraminidase
Pandemics
pandemic
Genes
Swine
genes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Angel, M., Kimble, J. B., Pena, L., Wan, H., & Perez, D. R. (2013). In vivo selection of H1N2 influenza virus reassortants in the Ferret model. Journal of Virology, 87(6), 3277-3283. https://doi.org/10.1128/JVI.02591-12

In vivo selection of H1N2 influenza virus reassortants in the Ferret model. / Angel, Matthew; Kimble, J. Brian; Pena, Lindomar; Wan, Hongquan; Perez, Daniel R.

In: Journal of Virology, Vol. 87, No. 6, 03.2013, p. 3277-3283.

Research output: Contribution to journalArticle

Angel, M, Kimble, JB, Pena, L, Wan, H & Perez, DR 2013, 'In vivo selection of H1N2 influenza virus reassortants in the Ferret model', Journal of Virology, vol. 87, no. 6, pp. 3277-3283. https://doi.org/10.1128/JVI.02591-12
Angel, Matthew ; Kimble, J. Brian ; Pena, Lindomar ; Wan, Hongquan ; Perez, Daniel R. / In vivo selection of H1N2 influenza virus reassortants in the Ferret model. In: Journal of Virology. 2013 ; Vol. 87, No. 6. pp. 3277-3283.
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