In vivo triglyceride synthesis in subcutaneous adipose tissue of humans correlates with plasma HDL parameters

Demidmaa Tuvdendorj, Alejandro O. Munoz, Viviana Ruiz-Barros, Jean Marc Schwarz, Giuseppe Montalto, Manisha Chandalia, Lawrence C. Sowers, Manfredi Rizzo, Elizabeth J. Murphy, Nicola Abate

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Backgrounds and aims: Low concentrations of plasma HDL-C are associated with the development of atherosclerotic cardiovascular diseases and type 2 diabetes. Here we aimed to explore the relationship between the in vivo fractional synthesis of triglycerides (fTG) in subcutaneous (s.q.) abdominal adipose tissue (AT), HDL-C concentrations and HDL particle size composition in non-diabetic humans. Methods: The fTG in s.q. abdominal AT was measured in 16 non-diabetic volunteers (7 women, 9 men; Age: 49 ± 20 years; BMI: 31 ± 5 kg/m; Fasting Plasma Glucose: 90 ± 10 mg/dl) after 2H2O labeling. HDL-C concentration and subclasses, large (L-HDL), intermediate (I-HDL) and small (S-HDL) were measured. Results: Linear regression analyses demonstrated significant associations of fTG with plasma concentration of HDL-C (r = 0.625,p = 0.009) and percent contribution of L-HDL (r = 0.798,p <0.001), I-HDL (r = -0.765,p <0.001) and S-HDL (r = -0.629, p = 0.009). When analyses were performed by gender, the associations remained significant in women (HDL-C: r = 0.822,p = 0.023; L-HDL: r = 0.892,p = 0.007; I-HDL: r = -0.927,p = 0.003) but not men. Conclusions: Our study demonstrated an in vivo association between subcutaneous abdominal adipose tissue lipid dynamics and HDL parameters in humans, but this was true for women not men. Positive association with L-HDL and negative with I-HDL suggest that subcutaneous abdominal adipose tissue lipid dynamics may play an important role in production of mature functional HDL particles. Further studies evaluating the mechanism responsible for these associations and the observed gender differences are important and warranted to identify potential novel targets of intervention to increase the production of atheroprotective subclasses of HDL-Cs and thus decreasing the risks of development of atherosclerotic conditions.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalAtherosclerosis
Volume251
DOIs
StatePublished - Aug 1 2016

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Abdominal Subcutaneous Fat
Subcutaneous Fat
Triglycerides
Lipids
Abdominal Fat
Particle Size
Type 2 Diabetes Mellitus
Volunteers
Linear Models
Fasting
Cardiovascular Diseases
Regression Analysis
Glucose

Keywords

  • Adipocyte
  • Atheroprotective
  • Gender-related
  • HDL-C metabolism
  • Mass spectrometry
  • Stable isotope tracer kinetics
  • Triglycerides

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

In vivo triglyceride synthesis in subcutaneous adipose tissue of humans correlates with plasma HDL parameters. / Tuvdendorj, Demidmaa; Munoz, Alejandro O.; Ruiz-Barros, Viviana; Schwarz, Jean Marc; Montalto, Giuseppe; Chandalia, Manisha; Sowers, Lawrence C.; Rizzo, Manfredi; Murphy, Elizabeth J.; Abate, Nicola.

In: Atherosclerosis, Vol. 251, 01.08.2016, p. 147-152.

Research output: Contribution to journalArticle

Tuvdendorj, D, Munoz, AO, Ruiz-Barros, V, Schwarz, JM, Montalto, G, Chandalia, M, Sowers, LC, Rizzo, M, Murphy, EJ & Abate, N 2016, 'In vivo triglyceride synthesis in subcutaneous adipose tissue of humans correlates with plasma HDL parameters', Atherosclerosis, vol. 251, pp. 147-152. https://doi.org/10.1016/j.atherosclerosis.2016.06.024
Tuvdendorj, Demidmaa ; Munoz, Alejandro O. ; Ruiz-Barros, Viviana ; Schwarz, Jean Marc ; Montalto, Giuseppe ; Chandalia, Manisha ; Sowers, Lawrence C. ; Rizzo, Manfredi ; Murphy, Elizabeth J. ; Abate, Nicola. / In vivo triglyceride synthesis in subcutaneous adipose tissue of humans correlates with plasma HDL parameters. In: Atherosclerosis. 2016 ; Vol. 251. pp. 147-152.
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abstract = "Backgrounds and aims: Low concentrations of plasma HDL-C are associated with the development of atherosclerotic cardiovascular diseases and type 2 diabetes. Here we aimed to explore the relationship between the in vivo fractional synthesis of triglycerides (fTG) in subcutaneous (s.q.) abdominal adipose tissue (AT), HDL-C concentrations and HDL particle size composition in non-diabetic humans. Methods: The fTG in s.q. abdominal AT was measured in 16 non-diabetic volunteers (7 women, 9 men; Age: 49 ± 20 years; BMI: 31 ± 5 kg/m; Fasting Plasma Glucose: 90 ± 10 mg/dl) after 2H2O labeling. HDL-C concentration and subclasses, large (L-HDL), intermediate (I-HDL) and small (S-HDL) were measured. Results: Linear regression analyses demonstrated significant associations of fTG with plasma concentration of HDL-C (r = 0.625,p = 0.009) and percent contribution of L-HDL (r = 0.798,p <0.001), I-HDL (r = -0.765,p <0.001) and S-HDL (r = -0.629, p = 0.009). When analyses were performed by gender, the associations remained significant in women (HDL-C: r = 0.822,p = 0.023; L-HDL: r = 0.892,p = 0.007; I-HDL: r = -0.927,p = 0.003) but not men. Conclusions: Our study demonstrated an in vivo association between subcutaneous abdominal adipose tissue lipid dynamics and HDL parameters in humans, but this was true for women not men. Positive association with L-HDL and negative with I-HDL suggest that subcutaneous abdominal adipose tissue lipid dynamics may play an important role in production of mature functional HDL particles. Further studies evaluating the mechanism responsible for these associations and the observed gender differences are important and warranted to identify potential novel targets of intervention to increase the production of atheroprotective subclasses of HDL-Cs and thus decreasing the risks of development of atherosclerotic conditions.",
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author = "Demidmaa Tuvdendorj and Munoz, {Alejandro O.} and Viviana Ruiz-Barros and Schwarz, {Jean Marc} and Giuseppe Montalto and Manisha Chandalia and Sowers, {Lawrence C.} and Manfredi Rizzo and Murphy, {Elizabeth J.} and Nicola Abate",
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AU - Tuvdendorj, Demidmaa

AU - Munoz, Alejandro O.

AU - Ruiz-Barros, Viviana

AU - Schwarz, Jean Marc

AU - Montalto, Giuseppe

AU - Chandalia, Manisha

AU - Sowers, Lawrence C.

AU - Rizzo, Manfredi

AU - Murphy, Elizabeth J.

AU - Abate, Nicola

PY - 2016/8/1

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N2 - Backgrounds and aims: Low concentrations of plasma HDL-C are associated with the development of atherosclerotic cardiovascular diseases and type 2 diabetes. Here we aimed to explore the relationship between the in vivo fractional synthesis of triglycerides (fTG) in subcutaneous (s.q.) abdominal adipose tissue (AT), HDL-C concentrations and HDL particle size composition in non-diabetic humans. Methods: The fTG in s.q. abdominal AT was measured in 16 non-diabetic volunteers (7 women, 9 men; Age: 49 ± 20 years; BMI: 31 ± 5 kg/m; Fasting Plasma Glucose: 90 ± 10 mg/dl) after 2H2O labeling. HDL-C concentration and subclasses, large (L-HDL), intermediate (I-HDL) and small (S-HDL) were measured. Results: Linear regression analyses demonstrated significant associations of fTG with plasma concentration of HDL-C (r = 0.625,p = 0.009) and percent contribution of L-HDL (r = 0.798,p <0.001), I-HDL (r = -0.765,p <0.001) and S-HDL (r = -0.629, p = 0.009). When analyses were performed by gender, the associations remained significant in women (HDL-C: r = 0.822,p = 0.023; L-HDL: r = 0.892,p = 0.007; I-HDL: r = -0.927,p = 0.003) but not men. Conclusions: Our study demonstrated an in vivo association between subcutaneous abdominal adipose tissue lipid dynamics and HDL parameters in humans, but this was true for women not men. Positive association with L-HDL and negative with I-HDL suggest that subcutaneous abdominal adipose tissue lipid dynamics may play an important role in production of mature functional HDL particles. Further studies evaluating the mechanism responsible for these associations and the observed gender differences are important and warranted to identify potential novel targets of intervention to increase the production of atheroprotective subclasses of HDL-Cs and thus decreasing the risks of development of atherosclerotic conditions.

AB - Backgrounds and aims: Low concentrations of plasma HDL-C are associated with the development of atherosclerotic cardiovascular diseases and type 2 diabetes. Here we aimed to explore the relationship between the in vivo fractional synthesis of triglycerides (fTG) in subcutaneous (s.q.) abdominal adipose tissue (AT), HDL-C concentrations and HDL particle size composition in non-diabetic humans. Methods: The fTG in s.q. abdominal AT was measured in 16 non-diabetic volunteers (7 women, 9 men; Age: 49 ± 20 years; BMI: 31 ± 5 kg/m; Fasting Plasma Glucose: 90 ± 10 mg/dl) after 2H2O labeling. HDL-C concentration and subclasses, large (L-HDL), intermediate (I-HDL) and small (S-HDL) were measured. Results: Linear regression analyses demonstrated significant associations of fTG with plasma concentration of HDL-C (r = 0.625,p = 0.009) and percent contribution of L-HDL (r = 0.798,p <0.001), I-HDL (r = -0.765,p <0.001) and S-HDL (r = -0.629, p = 0.009). When analyses were performed by gender, the associations remained significant in women (HDL-C: r = 0.822,p = 0.023; L-HDL: r = 0.892,p = 0.007; I-HDL: r = -0.927,p = 0.003) but not men. Conclusions: Our study demonstrated an in vivo association between subcutaneous abdominal adipose tissue lipid dynamics and HDL parameters in humans, but this was true for women not men. Positive association with L-HDL and negative with I-HDL suggest that subcutaneous abdominal adipose tissue lipid dynamics may play an important role in production of mature functional HDL particles. Further studies evaluating the mechanism responsible for these associations and the observed gender differences are important and warranted to identify potential novel targets of intervention to increase the production of atheroprotective subclasses of HDL-Cs and thus decreasing the risks of development of atherosclerotic conditions.

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KW - Atheroprotective

KW - Gender-related

KW - HDL-C metabolism

KW - Mass spectrometry

KW - Stable isotope tracer kinetics

KW - Triglycerides

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