Inactivation and modification of superoxide dismutase by glyoxal

Prevention by antibodies

Rukhsana Jabeen, M. Saleemuddin, John Petersen, Amin Mohammad

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glyoxal is an endogenous compound, the levels of which are increased in various pathologies associated with hyperglycaemia and other related disorders. It has been reported to inactivate critical cellular enzymes by promoting their cross-linking and perpetuates advanced glycation end-product (AGE) formation. In this study, we used superoxide dismutase (SOD) as a model to investigate the ability of specific anti-enzyme antibodies and monomer Fab fragments to protect against glyoxal-induced deactivation and aggregate formation. We found that glyoxal deactivated SOD, in a concentration and time-dependent fashion. The enzymatic activity was monitored spectrophotometrically and it was found that enzyme lost approximately 95% of its original activity, when exposed to 10 mM glyoxal for 120 h. SDS-polyacrylamide gel electrophoresis demonstrated the formation of high molecular weight aggregates in SOD samples exposed to glyoxal. Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) showed increase in relative molecular mass (Mr), upon exposure to glyoxal. Specific anti-enzyme antibodies and monomer Fab fragments markedly inhibited SOD deactivation caused by glyoxal and decreased the extent of cross-linking or formation of aggregates. This protection by the antibodies or Fab fragments was specific since, other non-specific antibodies were not able to protect SOD. Previously, antibodies have been used to prevent aggregation of β-amyloid peptides in Alzheimer and prion-protein disease. Our findings provide a new perspective, for use of antibodies to prevent the biomolecules against glycation-induced deactivation and alteration.

Original languageEnglish (US)
Pages (from-to)311-318
Number of pages8
JournalBiochimie
Volume89
Issue number3
DOIs
StatePublished - Mar 2007

Fingerprint

Glyoxal
Superoxide Dismutase
Antibodies
Immunoglobulin Fab Fragments
Enzymes
Anti-Idiotypic Antibodies
Monomers
Immunoglobulin Fragments
Advanced Glycosylation End Products
Prion Diseases
Molecular mass
Biomolecules
Pathology
Electrophoresis
Amyloid
Hyperglycemia
Ionization
Mass spectrometry
Polyacrylamide Gel Electrophoresis
Desorption

Keywords

  • Glyoxal
  • Maillard reaction
  • Polyclonal antibodies
  • SOD

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inactivation and modification of superoxide dismutase by glyoxal : Prevention by antibodies. / Jabeen, Rukhsana; Saleemuddin, M.; Petersen, John; Mohammad, Amin.

In: Biochimie, Vol. 89, No. 3, 03.2007, p. 311-318.

Research output: Contribution to journalArticle

Jabeen, Rukhsana ; Saleemuddin, M. ; Petersen, John ; Mohammad, Amin. / Inactivation and modification of superoxide dismutase by glyoxal : Prevention by antibodies. In: Biochimie. 2007 ; Vol. 89, No. 3. pp. 311-318.
@article{7a6097f190594a35a4798cf1daf77c92,
title = "Inactivation and modification of superoxide dismutase by glyoxal: Prevention by antibodies",
abstract = "Glyoxal is an endogenous compound, the levels of which are increased in various pathologies associated with hyperglycaemia and other related disorders. It has been reported to inactivate critical cellular enzymes by promoting their cross-linking and perpetuates advanced glycation end-product (AGE) formation. In this study, we used superoxide dismutase (SOD) as a model to investigate the ability of specific anti-enzyme antibodies and monomer Fab fragments to protect against glyoxal-induced deactivation and aggregate formation. We found that glyoxal deactivated SOD, in a concentration and time-dependent fashion. The enzymatic activity was monitored spectrophotometrically and it was found that enzyme lost approximately 95{\%} of its original activity, when exposed to 10 mM glyoxal for 120 h. SDS-polyacrylamide gel electrophoresis demonstrated the formation of high molecular weight aggregates in SOD samples exposed to glyoxal. Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) showed increase in relative molecular mass (Mr), upon exposure to glyoxal. Specific anti-enzyme antibodies and monomer Fab fragments markedly inhibited SOD deactivation caused by glyoxal and decreased the extent of cross-linking or formation of aggregates. This protection by the antibodies or Fab fragments was specific since, other non-specific antibodies were not able to protect SOD. Previously, antibodies have been used to prevent aggregation of β-amyloid peptides in Alzheimer and prion-protein disease. Our findings provide a new perspective, for use of antibodies to prevent the biomolecules against glycation-induced deactivation and alteration.",
keywords = "Glyoxal, Maillard reaction, Polyclonal antibodies, SOD",
author = "Rukhsana Jabeen and M. Saleemuddin and John Petersen and Amin Mohammad",
year = "2007",
month = "3",
doi = "10.1016/j.biochi.2006.10.015",
language = "English (US)",
volume = "89",
pages = "311--318",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Inactivation and modification of superoxide dismutase by glyoxal

T2 - Prevention by antibodies

AU - Jabeen, Rukhsana

AU - Saleemuddin, M.

AU - Petersen, John

AU - Mohammad, Amin

PY - 2007/3

Y1 - 2007/3

N2 - Glyoxal is an endogenous compound, the levels of which are increased in various pathologies associated with hyperglycaemia and other related disorders. It has been reported to inactivate critical cellular enzymes by promoting their cross-linking and perpetuates advanced glycation end-product (AGE) formation. In this study, we used superoxide dismutase (SOD) as a model to investigate the ability of specific anti-enzyme antibodies and monomer Fab fragments to protect against glyoxal-induced deactivation and aggregate formation. We found that glyoxal deactivated SOD, in a concentration and time-dependent fashion. The enzymatic activity was monitored spectrophotometrically and it was found that enzyme lost approximately 95% of its original activity, when exposed to 10 mM glyoxal for 120 h. SDS-polyacrylamide gel electrophoresis demonstrated the formation of high molecular weight aggregates in SOD samples exposed to glyoxal. Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) showed increase in relative molecular mass (Mr), upon exposure to glyoxal. Specific anti-enzyme antibodies and monomer Fab fragments markedly inhibited SOD deactivation caused by glyoxal and decreased the extent of cross-linking or formation of aggregates. This protection by the antibodies or Fab fragments was specific since, other non-specific antibodies were not able to protect SOD. Previously, antibodies have been used to prevent aggregation of β-amyloid peptides in Alzheimer and prion-protein disease. Our findings provide a new perspective, for use of antibodies to prevent the biomolecules against glycation-induced deactivation and alteration.

AB - Glyoxal is an endogenous compound, the levels of which are increased in various pathologies associated with hyperglycaemia and other related disorders. It has been reported to inactivate critical cellular enzymes by promoting their cross-linking and perpetuates advanced glycation end-product (AGE) formation. In this study, we used superoxide dismutase (SOD) as a model to investigate the ability of specific anti-enzyme antibodies and monomer Fab fragments to protect against glyoxal-induced deactivation and aggregate formation. We found that glyoxal deactivated SOD, in a concentration and time-dependent fashion. The enzymatic activity was monitored spectrophotometrically and it was found that enzyme lost approximately 95% of its original activity, when exposed to 10 mM glyoxal for 120 h. SDS-polyacrylamide gel electrophoresis demonstrated the formation of high molecular weight aggregates in SOD samples exposed to glyoxal. Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) showed increase in relative molecular mass (Mr), upon exposure to glyoxal. Specific anti-enzyme antibodies and monomer Fab fragments markedly inhibited SOD deactivation caused by glyoxal and decreased the extent of cross-linking or formation of aggregates. This protection by the antibodies or Fab fragments was specific since, other non-specific antibodies were not able to protect SOD. Previously, antibodies have been used to prevent aggregation of β-amyloid peptides in Alzheimer and prion-protein disease. Our findings provide a new perspective, for use of antibodies to prevent the biomolecules against glycation-induced deactivation and alteration.

KW - Glyoxal

KW - Maillard reaction

KW - Polyclonal antibodies

KW - SOD

UR - http://www.scopus.com/inward/record.url?scp=33947371321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947371321&partnerID=8YFLogxK

U2 - 10.1016/j.biochi.2006.10.015

DO - 10.1016/j.biochi.2006.10.015

M3 - Article

VL - 89

SP - 311

EP - 318

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 3

ER -