Inactivation of plasma α1-proteinase inhibitor by acrolein

Adduct formation with lysine and histidine residues

J. C. Gan, Ghulam Ansari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Four new peaks were observed upon amino acid analysis of α1-proteinase inhibitor (α1-PI), which was inactivated by acrolein under in vitro conditions. The first peak emerged just before ammonia, the second and third between ammonia and lysine, and the fourth between histidine and arginine. The new fourth peak was also observed when model compounds of lysine (N-acetyllysine or polylysine) were reacted with acrolein and subsequently processed for amino acid analysis. This new compound was purified by high-voltage paper electrophoresis and subjected to fast atom bombardment mass spectrometry, which showed a protonated molecule ion at m/z 203 [M + H]+ followed by m/z 186 [M + H+ - NH3]+. This compound was thus identified as 3-oxopropyllysine, a lysine adduct of acrolein. Similarly, when a model polypeptide of histidine, polyhistidine, was reacted with acrolein under the same conditions as α1-PI, three new peaks (besides histidine) emerged from the column. Their elution times corresponded to the first three new peaks found in the hydrolysates of acrolein treated α1-PI.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalMolecular Toxicology
Volume2
Issue number3
StatePublished - 1989

Fingerprint

Acrolein
Histidine
Lysine
Peptide Hydrolases
Plasmas
Ammonia
Fast Atom Bombardment Mass Spectrometry
Paper Electrophoresis
Amino Acids
Polylysine
Electrophoresis
Mass spectrometry
Arginine
Ions
Atoms
Peptides
Molecules
Electric potential

ASJC Scopus subject areas

  • Toxicology

Cite this

Inactivation of plasma α1-proteinase inhibitor by acrolein : Adduct formation with lysine and histidine residues. / Gan, J. C.; Ansari, Ghulam.

In: Molecular Toxicology, Vol. 2, No. 3, 1989, p. 137-145.

Research output: Contribution to journalArticle

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