Inactivity amplifies the catabolic response of skeletal muscle to cortisol

Arny A. Ferrando, Charles A. Stuart, Melinda Sheffield-Moore, Robert R. Wolfe

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Severe injury or trauma is accompanied by both hypercortisolemia and prolonged inactivity or bed rest (BR). Trauma and BR alone each result in a loss of muscle nitrogen, albeit through different metabolic alterations. Although BR alone can result in a 2-3% loss of lean body mass, the effects of severe trauma can be 2- to 3-fold greater. We investigated the combined effects of hypercortisolemia and prolonged inactivity on muscle protein metabolism in healthy volunteers. Six males were studied before and after 14 days of strict BR using a model based on arteriovenous sampling and muscle biopsy. Fractional synthesis and breakdown rates of skeletal muscle protein were also directly calculated. Each assessment of protein metabolism was conducted during a 12-h infusion of hydrocortisone sodium succinate (120 μg/kg·h), resulting in blood cortisol concentrations that mimic severe injury (≃31 μg/dL). After 14 days of strict BR, hypercortisolemia increased phenylalanine efflux from muscle by 3-fold (P < 0.05). The augmented negative amino acid balance was the result of an increased muscle protein breakdown (P < 0.05) without a concomitant change in muscle protein synthesis. Muscle efflux of glutamine and alanine increased significantly after bed rest due to a significant increase in de novo synthesis (P < 0.05). Thus, inactivity sensitizes skeletal muscle to the catabolic effects of hypercortisolemia. Furthermore, these effects on healthy volunteers are analogous to those seen after severe injury.

Original languageEnglish
Pages (from-to)3515-3521
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume84
Issue number10
StatePublished - 1999

Fingerprint

Bed Rest
Muscle Proteins
Muscle
Hydrocortisone
Skeletal Muscle
Wounds and Injuries
Muscles
Metabolism
Healthy Volunteers
Biopsy
Succinic Acid
Glutamine
Phenylalanine
Alanine
Blood
Nitrogen
Sodium
Sampling
Amino Acids
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Ferrando, A. A., Stuart, C. A., Sheffield-Moore, M., & Wolfe, R. R. (1999). Inactivity amplifies the catabolic response of skeletal muscle to cortisol. Journal of Clinical Endocrinology and Metabolism, 84(10), 3515-3521.

Inactivity amplifies the catabolic response of skeletal muscle to cortisol. / Ferrando, Arny A.; Stuart, Charles A.; Sheffield-Moore, Melinda; Wolfe, Robert R.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 84, No. 10, 1999, p. 3515-3521.

Research output: Contribution to journalArticle

Ferrando, AA, Stuart, CA, Sheffield-Moore, M & Wolfe, RR 1999, 'Inactivity amplifies the catabolic response of skeletal muscle to cortisol', Journal of Clinical Endocrinology and Metabolism, vol. 84, no. 10, pp. 3515-3521.
Ferrando, Arny A. ; Stuart, Charles A. ; Sheffield-Moore, Melinda ; Wolfe, Robert R. / Inactivity amplifies the catabolic response of skeletal muscle to cortisol. In: Journal of Clinical Endocrinology and Metabolism. 1999 ; Vol. 84, No. 10. pp. 3515-3521.
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