Increased colonic apelin production in rodents with experimental colitis and in humans with IBD

Song Han, Guiyun Wang, Suimin Qiu, Carol de la Motte, Hui Qun Wang, Guillermo Gomez, Ella Englander, George H. Greeley

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Apelin and its receptor, the APJ receptor, are expressed in the gastrointestinal tract. The aims of this study were to examine the effects of sodium dextran sulfate (DSS)-induced experimental colitis in rats and mice and inflammatory bowel disease (IBD) in humans on intestinal apelin production, and the influence of exogenous apelin on colonic epithelial cell proliferation in mice. In rodents with experimental colitis, colonic apelin mRNA levels were elevated during the inflammatory reaction as well as during the tissue repair phase that ensues after DSS withdrawal. Fluctuations in colonic apelin expression were paralleled by similar changes in apelin immunostaining. Apelin immunostaining was increased in the surface epithelium, in epithelial cells along the length of the tubular gland and in the stem cell region at the gland base. In ulcerative colitis (UC) and Crohn's disease patients, apelin immunostaining revealed a pattern of increased intestinal apelin content similar to that observed in rodents with experimental colitis. Administration of synthetic apelin to mice during the recovery phase of DSS-induced colitis stimulated colonic epithelial cell proliferation significantly. Our observations that colonic apelin production is increased during and after DSS exposure indicate that apelin plays multiple roles during the different stages of colitis. Additionally, the stimulatory action of exogenous apelin on colonic epithelial proliferation suggests that the increased apelin production during intestinal recovery stage may contribute to the repair of the intestinal epithelium in experimental rodent models of colitis and in IBD patients.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalRegulatory Peptides
Volume142
Issue number3
DOIs
StatePublished - Aug 16 2007

Fingerprint

Colitis
Inflammatory Bowel Diseases
Rodentia
Cell proliferation
Repair
Recovery
Dextran Sulfate
Epithelial Cells
Stem cells
Rats
Cell Proliferation
Tissue
Messenger RNA
Gastrointestinal Contents
Intestinal Mucosa
Ulcerative Colitis
Crohn Disease
Gastrointestinal Tract
Theoretical Models
Stem Cells

Keywords

  • Crohn's
  • Expression
  • Immunostaining
  • Mice
  • mRNA
  • Rats

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Increased colonic apelin production in rodents with experimental colitis and in humans with IBD. / Han, Song; Wang, Guiyun; Qiu, Suimin; de la Motte, Carol; Wang, Hui Qun; Gomez, Guillermo; Englander, Ella; Greeley, George H.

In: Regulatory Peptides, Vol. 142, No. 3, 16.08.2007, p. 131-137.

Research output: Contribution to journalArticle

Han, Song ; Wang, Guiyun ; Qiu, Suimin ; de la Motte, Carol ; Wang, Hui Qun ; Gomez, Guillermo ; Englander, Ella ; Greeley, George H. / Increased colonic apelin production in rodents with experimental colitis and in humans with IBD. In: Regulatory Peptides. 2007 ; Vol. 142, No. 3. pp. 131-137.
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