Increased concentration of high-mobility group box 1 protein in milk is related to the severity of bovine mastitis

Yuki Furukawa, Tomohito Hayashi, Midori Mizuta, Shota Ebara, Yoshio Kiku, Tomomi Ozawa, Tomoko Matsubara, Ichiaki Ito, Daisuke Kitamura, Ryushin Mizuta

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

High-mobility group box 1 (HMGB1) protein is the major component of the nonhistone nuclear protein group and is involved in nucleosome stabilization and transcription regulation. HMGB1 has recently been focused on as a proinflammatory cytokine associated with various inflammatory diseases and as a target of anti-inflammatory therapy. Mastitis, a serious inflammatory disease of dairy cows, is caused by infection of the mammary gland and has detrimental effects on the quantity and quality of milk. By detecting the presence of HMGB1 in milk, we investigated the correlation between HMGB1 concentration and the severity of bovine mastitis, which was determined using the California Mastitis Test and somatic cell count (SCC). We detected a substantial amount of HMGB1 in mastitic milk but not in the milk from normal cows. We used the Spearman rank correlation coefficient to assess the relationship between HMGB1 concentration and SCC and found a significant correlation (n = 12, r = 0.975). Thus, we confirmed the positive correlation between HMGB1 concentration and SCC in milk, i.e., the severity of mastitis, which suggested that HMGB1 in milk is a new indicator of bovine mastitis.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalVeterinary Research Communications
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Fingerprint

Bovine Mastitis
HMGB1 Protein
bovine mastitis
dairy protein
Milk
milk
somatic cell count
Mastitis
Cell Count
mastitis
nucleosomes
milk quality
nuclear proteins
Nucleosomes
mammary glands
Human Mammary Glands
Nonparametric Statistics
Nuclear Proteins
cytokines
dairy cows

Keywords

  • Bovine mastitis
  • High-mobility-group box 1
  • Infection
  • Inflammation

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Increased concentration of high-mobility group box 1 protein in milk is related to the severity of bovine mastitis. / Furukawa, Yuki; Hayashi, Tomohito; Mizuta, Midori; Ebara, Shota; Kiku, Yoshio; Ozawa, Tomomi; Matsubara, Tomoko; Ito, Ichiaki; Kitamura, Daisuke; Mizuta, Ryushin.

In: Veterinary Research Communications, Vol. 35, No. 1, 01.01.2011, p. 47-54.

Research output: Contribution to journalArticle

Furukawa, Y, Hayashi, T, Mizuta, M, Ebara, S, Kiku, Y, Ozawa, T, Matsubara, T, Ito, I, Kitamura, D & Mizuta, R 2011, 'Increased concentration of high-mobility group box 1 protein in milk is related to the severity of bovine mastitis', Veterinary Research Communications, vol. 35, no. 1, pp. 47-54. https://doi.org/10.1007/s11259-010-9454-6
Furukawa, Yuki ; Hayashi, Tomohito ; Mizuta, Midori ; Ebara, Shota ; Kiku, Yoshio ; Ozawa, Tomomi ; Matsubara, Tomoko ; Ito, Ichiaki ; Kitamura, Daisuke ; Mizuta, Ryushin. / Increased concentration of high-mobility group box 1 protein in milk is related to the severity of bovine mastitis. In: Veterinary Research Communications. 2011 ; Vol. 35, No. 1. pp. 47-54.
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