Increased expression of high affinity ige (FcεRI) receptor-α chain mRNA and protein-bearing eosinophils in human allergen-induced atopic asthma

K. Rajakulasingam, S. Till, S. Ying, M. Humbert, J. Barkans, M. Sullivan, Q. Meng, C. J. Corrigan, J. Bungre, J. A. Grant, A. B. Kay, S. R. Durham

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Abstract

FcεRI receptors play an important role in allergen-induced mediator release and antigen presentation by mast cells, basophils, and monocyte/macrophages in atopic disorders. The expression of FcεRI by tissue eosinophils in atopic asthma after allergen challenge has not been established. For this reason we attempted to identify mRNA and protein product + FcεRIα eosinophils in cytospins made from bronchoalveolar lavage (BAL) from atopic asthmatics (n = 9) and nonatopic normal subjects (n = 4) 24 h after segmental challenge with allergen or diluent. Messenger RNA for FcεRIα was determined using in situ hybridization and FcεRIα protein expression by immunocytochemistry using a mouse monoclonal antibody 22E7. Colocalization of FcεRIα receptors to eosinophils was performed using chromotrope 2R. When compared with a control challenge, segmental challenge with Dermatophagoides pteronyssinus induced significant BAL eosinophilia (p = 0.007). The total number of BAL FcεRIα mRNA and protein-positive cells also increased in asthmatics, median values 2 (0.7-7.2) and 11.5 (0.6-65.0) X 106 cells (p = 0.02) and 0 (0-0.3 x 106) and 3.1 x 106 (0.45 - 162.5 x 106) cells (p = 0.007), respectively, for mRNA and protein. Net increases in FcεRIα+ cells correlated with the net increases in BAL eosinophils (r = 0.98, p = 0.0001 for mRNA and r = 0.72, p = 0.02 for protein). Colocalization studies with chromotrope 2R revealed that only 4% of FcεRIα+ cells were eosinophils after control challenge and; in contrast, 85 to 95% of FcεRIα+ cells were eosinophils after allergen. There were no differences in the numbers of FcεRIα+ cells or eosinophils in normal control subjects. Our results demonstrated that local endobronchial allergen provocation in atopic asthmatics results in increased synthesis and expression of FcεRIα predominantly on BAL eosinophils.

Original languageEnglish (US)
Pages (from-to)233-240
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume158
Issue number1
StatePublished - 1998

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Eosinophils
Allergens
Asthma
Bronchoalveolar Lavage
Messenger RNA
Proteins
Dermatophagoides pteronyssinus
Basophils
Antigen Presentation
Eosinophilia
Mast Cells
In Situ Hybridization
Monocytes
Cell Count
Immunohistochemistry
Macrophages
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Increased expression of high affinity ige (FcεRI) receptor-α chain mRNA and protein-bearing eosinophils in human allergen-induced atopic asthma. / Rajakulasingam, K.; Till, S.; Ying, S.; Humbert, M.; Barkans, J.; Sullivan, M.; Meng, Q.; Corrigan, C. J.; Bungre, J.; Grant, J. A.; Kay, A. B.; Durham, S. R.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 158, No. 1, 1998, p. 233-240.

Research output: Contribution to journalArticle

Rajakulasingam, K, Till, S, Ying, S, Humbert, M, Barkans, J, Sullivan, M, Meng, Q, Corrigan, CJ, Bungre, J, Grant, JA, Kay, AB & Durham, SR 1998, 'Increased expression of high affinity ige (FcεRI) receptor-α chain mRNA and protein-bearing eosinophils in human allergen-induced atopic asthma', American Journal of Respiratory and Critical Care Medicine, vol. 158, no. 1, pp. 233-240.
Rajakulasingam, K. ; Till, S. ; Ying, S. ; Humbert, M. ; Barkans, J. ; Sullivan, M. ; Meng, Q. ; Corrigan, C. J. ; Bungre, J. ; Grant, J. A. ; Kay, A. B. ; Durham, S. R. / Increased expression of high affinity ige (FcεRI) receptor-α chain mRNA and protein-bearing eosinophils in human allergen-induced atopic asthma. In: American Journal of Respiratory and Critical Care Medicine. 1998 ; Vol. 158, No. 1. pp. 233-240.
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abstract = "FcεRI receptors play an important role in allergen-induced mediator release and antigen presentation by mast cells, basophils, and monocyte/macrophages in atopic disorders. The expression of FcεRI by tissue eosinophils in atopic asthma after allergen challenge has not been established. For this reason we attempted to identify mRNA and protein product + FcεRIα eosinophils in cytospins made from bronchoalveolar lavage (BAL) from atopic asthmatics (n = 9) and nonatopic normal subjects (n = 4) 24 h after segmental challenge with allergen or diluent. Messenger RNA for FcεRIα was determined using in situ hybridization and FcεRIα protein expression by immunocytochemistry using a mouse monoclonal antibody 22E7. Colocalization of FcεRIα receptors to eosinophils was performed using chromotrope 2R. When compared with a control challenge, segmental challenge with Dermatophagoides pteronyssinus induced significant BAL eosinophilia (p = 0.007). The total number of BAL FcεRIα mRNA and protein-positive cells also increased in asthmatics, median values 2 (0.7-7.2) and 11.5 (0.6-65.0) X 106 cells (p = 0.02) and 0 (0-0.3 x 106) and 3.1 x 106 (0.45 - 162.5 x 106) cells (p = 0.007), respectively, for mRNA and protein. Net increases in FcεRIα+ cells correlated with the net increases in BAL eosinophils (r = 0.98, p = 0.0001 for mRNA and r = 0.72, p = 0.02 for protein). Colocalization studies with chromotrope 2R revealed that only 4{\%} of FcεRIα+ cells were eosinophils after control challenge and; in contrast, 85 to 95{\%} of FcεRIα+ cells were eosinophils after allergen. There were no differences in the numbers of FcεRIα+ cells or eosinophils in normal control subjects. Our results demonstrated that local endobronchial allergen provocation in atopic asthmatics results in increased synthesis and expression of FcεRIα predominantly on BAL eosinophils.",
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AU - Rajakulasingam, K.

AU - Till, S.

AU - Ying, S.

AU - Humbert, M.

AU - Barkans, J.

AU - Sullivan, M.

AU - Meng, Q.

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AU - Grant, J. A.

AU - Kay, A. B.

AU - Durham, S. R.

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N2 - FcεRI receptors play an important role in allergen-induced mediator release and antigen presentation by mast cells, basophils, and monocyte/macrophages in atopic disorders. The expression of FcεRI by tissue eosinophils in atopic asthma after allergen challenge has not been established. For this reason we attempted to identify mRNA and protein product + FcεRIα eosinophils in cytospins made from bronchoalveolar lavage (BAL) from atopic asthmatics (n = 9) and nonatopic normal subjects (n = 4) 24 h after segmental challenge with allergen or diluent. Messenger RNA for FcεRIα was determined using in situ hybridization and FcεRIα protein expression by immunocytochemistry using a mouse monoclonal antibody 22E7. Colocalization of FcεRIα receptors to eosinophils was performed using chromotrope 2R. When compared with a control challenge, segmental challenge with Dermatophagoides pteronyssinus induced significant BAL eosinophilia (p = 0.007). The total number of BAL FcεRIα mRNA and protein-positive cells also increased in asthmatics, median values 2 (0.7-7.2) and 11.5 (0.6-65.0) X 106 cells (p = 0.02) and 0 (0-0.3 x 106) and 3.1 x 106 (0.45 - 162.5 x 106) cells (p = 0.007), respectively, for mRNA and protein. Net increases in FcεRIα+ cells correlated with the net increases in BAL eosinophils (r = 0.98, p = 0.0001 for mRNA and r = 0.72, p = 0.02 for protein). Colocalization studies with chromotrope 2R revealed that only 4% of FcεRIα+ cells were eosinophils after control challenge and; in contrast, 85 to 95% of FcεRIα+ cells were eosinophils after allergen. There were no differences in the numbers of FcεRIα+ cells or eosinophils in normal control subjects. Our results demonstrated that local endobronchial allergen provocation in atopic asthmatics results in increased synthesis and expression of FcεRIα predominantly on BAL eosinophils.

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