FcεRI receptors play an important role in allergen-induced mediator release and antigen presentation by mast cells, basophils, and monocyte/macrophages in atopic disorders. The expression of FcεRI by tissue eosinophils in atopic asthma after allergen challenge has not been established. For this reason we attempted to identify mRNA and protein product + FcεRIα eosinophils in cytospins made from bronchoalveolar lavage (BAL) from atopic asthmatics (n = 9) and nonatopic normal subjects (n = 4) 24 h after segmental challenge with allergen or diluent. Messenger RNA for FcεRIα was determined using in situ hybridization and FcεRIα protein expression by immunocytochemistry using a mouse monoclonal antibody 22E7. Colocalization of FcεRIα receptors to eosinophils was performed using chromotrope 2R. When compared with a control challenge, segmental challenge with Dermatophagoides pteronyssinus induced significant BAL eosinophilia (p = 0.007). The total number of BAL FcεRIα mRNA and protein-positive cells also increased in asthmatics, median values 2 (0.7-7.2) and 11.5 (0.6-65.0) X 106 cells (p = 0.02) and 0 (0-0.3 x 106) and 3.1 x 106 (0.45 - 162.5 x 106) cells (p = 0.007), respectively, for mRNA and protein. Net increases in FcεRIα+ cells correlated with the net increases in BAL eosinophils (r = 0.98, p = 0.0001 for mRNA and r = 0.72, p = 0.02 for protein). Colocalization studies with chromotrope 2R revealed that only 4% of FcεRIα+ cells were eosinophils after control challenge and; in contrast, 85 to 95% of FcεRIα+ cells were eosinophils after allergen. There were no differences in the numbers of FcεRIα+ cells or eosinophils in normal control subjects. Our results demonstrated that local endobronchial allergen provocation in atopic asthmatics results in increased synthesis and expression of FcεRIα predominantly on BAL eosinophils.
|Original language||English (US)|
|Number of pages||8|
|Journal||American Journal of Respiratory and Critical Care Medicine|
|State||Published - 1998|
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Critical Care and Intensive Care Medicine