Increased expression of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage and P450 17α-hydroxylase enzymes in ovarian hyperthecosis

Manubai Nagamani, Randall Urban

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To investigate whether the increased ovarian androgen synthesis in hyperthecosis is due to increased expression of the steroidogenic enzymes essential for androgen synthesis. Design: Controlled study to investigate concentration of steroidogenic enzymes in the ovarian stroma of women with hyperthecosis of the ovaries. Setting: Academic research environment. Patient(s): Three women with ovarian hyperthecosis and eight with normal ovulatory cycles. Intervention(s): Ovarian stromal tissues were obtained from women with hyperthecosis and women with normal ovaries. Diagnosis of hyperthecosis was confirmed by histologic examination of the ovaries. Steroid levels were measured in the ovarian vein serum of one patient with hyperthecosis. Main Outcome Measure(s): Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled complementary DNA (cDNA) probes encoding human P450(scc) and P450(17α) enzymes. Result(s): P450(scc) and P45017(α) messenger RNAs (mRNAs) were detected in the normal ovarian stroma and stromal hyperthecosis. Compared with normal ovarian stroma, P450(scc) mRNA was increased twofold and P450(17α) mRNA was increased threefold in stromal hyperthecosis. Conclusion(s): [1] Ovarian stroma is probably the site of androgen production in ovarian hyperthecosis. [2] Increased stromal androgen synthesis in hyperthecosis could be due to increased expression of the enzymes P450(scc) and P450(17α) in the ovarian stroma. [3] Markedly increased concentrations of 17α-hydroxyprogesterone in the ovarian vein serum indicate possible dysregulation of P450(17α) in ovarian hyperthecosis.

Original languageEnglish (US)
Pages (from-to)328-333
Number of pages6
JournalFertility and Sterility
Volume71
Issue number2
DOIs
StatePublished - Feb 1999

Fingerprint

Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Androgens
Cholesterol
RNA
Ovary
Enzymes
Messenger RNA
Veins
17-alpha-Hydroxyprogesterone
DNA Probes
Serum
Northern Blotting
Nitrogen
Complementary DNA
Steroids
Outcome Assessment (Health Care)
Research

Keywords

  • 17α-hydroxyprogesterone
  • Hyperthecosis
  • P450 17α-hydroxylase enzyme
  • P450 side-chain cleavage enzyme

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

@article{60fe433ba66248189935328c7a8f5193,
title = "Increased expression of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage and P450 17α-hydroxylase enzymes in ovarian hyperthecosis",
abstract = "Objective: To investigate whether the increased ovarian androgen synthesis in hyperthecosis is due to increased expression of the steroidogenic enzymes essential for androgen synthesis. Design: Controlled study to investigate concentration of steroidogenic enzymes in the ovarian stroma of women with hyperthecosis of the ovaries. Setting: Academic research environment. Patient(s): Three women with ovarian hyperthecosis and eight with normal ovulatory cycles. Intervention(s): Ovarian stromal tissues were obtained from women with hyperthecosis and women with normal ovaries. Diagnosis of hyperthecosis was confirmed by histologic examination of the ovaries. Steroid levels were measured in the ovarian vein serum of one patient with hyperthecosis. Main Outcome Measure(s): Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled complementary DNA (cDNA) probes encoding human P450(scc) and P450(17α) enzymes. Result(s): P450(scc) and P45017(α) messenger RNAs (mRNAs) were detected in the normal ovarian stroma and stromal hyperthecosis. Compared with normal ovarian stroma, P450(scc) mRNA was increased twofold and P450(17α) mRNA was increased threefold in stromal hyperthecosis. Conclusion(s): [1] Ovarian stroma is probably the site of androgen production in ovarian hyperthecosis. [2] Increased stromal androgen synthesis in hyperthecosis could be due to increased expression of the enzymes P450(scc) and P450(17α) in the ovarian stroma. [3] Markedly increased concentrations of 17α-hydroxyprogesterone in the ovarian vein serum indicate possible dysregulation of P450(17α) in ovarian hyperthecosis.",
keywords = "17α-hydroxyprogesterone, Hyperthecosis, P450 17α-hydroxylase enzyme, P450 side-chain cleavage enzyme",
author = "Manubai Nagamani and Randall Urban",
year = "1999",
month = "2",
doi = "10.1016/S0015-0282(98)00464-6",
language = "English (US)",
volume = "71",
pages = "328--333",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Increased expression of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage and P450 17α-hydroxylase enzymes in ovarian hyperthecosis

AU - Nagamani, Manubai

AU - Urban, Randall

PY - 1999/2

Y1 - 1999/2

N2 - Objective: To investigate whether the increased ovarian androgen synthesis in hyperthecosis is due to increased expression of the steroidogenic enzymes essential for androgen synthesis. Design: Controlled study to investigate concentration of steroidogenic enzymes in the ovarian stroma of women with hyperthecosis of the ovaries. Setting: Academic research environment. Patient(s): Three women with ovarian hyperthecosis and eight with normal ovulatory cycles. Intervention(s): Ovarian stromal tissues were obtained from women with hyperthecosis and women with normal ovaries. Diagnosis of hyperthecosis was confirmed by histologic examination of the ovaries. Steroid levels were measured in the ovarian vein serum of one patient with hyperthecosis. Main Outcome Measure(s): Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled complementary DNA (cDNA) probes encoding human P450(scc) and P450(17α) enzymes. Result(s): P450(scc) and P45017(α) messenger RNAs (mRNAs) were detected in the normal ovarian stroma and stromal hyperthecosis. Compared with normal ovarian stroma, P450(scc) mRNA was increased twofold and P450(17α) mRNA was increased threefold in stromal hyperthecosis. Conclusion(s): [1] Ovarian stroma is probably the site of androgen production in ovarian hyperthecosis. [2] Increased stromal androgen synthesis in hyperthecosis could be due to increased expression of the enzymes P450(scc) and P450(17α) in the ovarian stroma. [3] Markedly increased concentrations of 17α-hydroxyprogesterone in the ovarian vein serum indicate possible dysregulation of P450(17α) in ovarian hyperthecosis.

AB - Objective: To investigate whether the increased ovarian androgen synthesis in hyperthecosis is due to increased expression of the steroidogenic enzymes essential for androgen synthesis. Design: Controlled study to investigate concentration of steroidogenic enzymes in the ovarian stroma of women with hyperthecosis of the ovaries. Setting: Academic research environment. Patient(s): Three women with ovarian hyperthecosis and eight with normal ovulatory cycles. Intervention(s): Ovarian stromal tissues were obtained from women with hyperthecosis and women with normal ovaries. Diagnosis of hyperthecosis was confirmed by histologic examination of the ovaries. Steroid levels were measured in the ovarian vein serum of one patient with hyperthecosis. Main Outcome Measure(s): Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled complementary DNA (cDNA) probes encoding human P450(scc) and P450(17α) enzymes. Result(s): P450(scc) and P45017(α) messenger RNAs (mRNAs) were detected in the normal ovarian stroma and stromal hyperthecosis. Compared with normal ovarian stroma, P450(scc) mRNA was increased twofold and P450(17α) mRNA was increased threefold in stromal hyperthecosis. Conclusion(s): [1] Ovarian stroma is probably the site of androgen production in ovarian hyperthecosis. [2] Increased stromal androgen synthesis in hyperthecosis could be due to increased expression of the enzymes P450(scc) and P450(17α) in the ovarian stroma. [3] Markedly increased concentrations of 17α-hydroxyprogesterone in the ovarian vein serum indicate possible dysregulation of P450(17α) in ovarian hyperthecosis.

KW - 17α-hydroxyprogesterone

KW - Hyperthecosis

KW - P450 17α-hydroxylase enzyme

KW - P450 side-chain cleavage enzyme

UR - http://www.scopus.com/inward/record.url?scp=0032908555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032908555&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(98)00464-6

DO - 10.1016/S0015-0282(98)00464-6

M3 - Article

C2 - 9988407

AN - SCOPUS:0032908555

VL - 71

SP - 328

EP - 333

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 2

ER -