Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice

Bill Ameredes, Ruben Zamora, Kevin F. Gibson, Timothy R. Billiar, Barbara Dixon-McCarthy, Simon Watkins, William Calhoun

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The anti-inflammatory cytokine interleukin (IL)-10 suppresses inducible nitric oxide synthase (iNOS); therefore, NO production should increase in the absence of IL-10. Production of NO (as nitrite) by bronchoalveolar lavage cells of IL-10 knockout (-/-) mice was assessed after ovalbumin sensitization and airway challenge (S/C) and was compared with the IL-10-sufficient, wild-type (WT) C57B6. Eosinophil recruitment occurred in S/C WT and IL-10-/- mice, suggesting allergic airway inflammation. Alveolar macrophages (per g mouse) were unchanged (∼3×104 cells) with the exception of a doubling in the S/C IL-10-/- mice (∼6×104 cells, P<0.05). NO production (per million cells) was doubled in cells from S/C IL-10-/- (15.3 μM) mice compared with WT (7.6 μM, P<0.05). Inhibition of iNOS by L-N5-(l-iminoethyl)-ornithine reduced NO production in all S/C mice, confirming that the increase was a result of up-regulation of iNOS. We conclude that IL-10 is a critical cytokine regulating iNOS in murine airway cells and that its absence can lead to up-regulation of iNOS and development of allergic airway inflammation.

Original languageEnglish (US)
Pages (from-to)730-736
Number of pages7
JournalJournal of Leukocyte Biology
Volume70
Issue number5
StatePublished - 2001
Externally publishedYes

Fingerprint

Knockout Mice
Interleukin-10
Nitric Oxide
Nitric Oxide Synthase Type II
Up-Regulation
Cytokines
Inflammation
Ornithine
Ovalbumin
Alveolar Macrophages
Bronchoalveolar Lavage
Nitrites
Eosinophils
Anti-Inflammatory Agents

Keywords

  • Allergen
  • Bronchoalveolar lavage
  • C57BL6 mice
  • Macrophage
  • Nitrite
  • Ovalbumin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ameredes, B., Zamora, R., Gibson, K. F., Billiar, T. R., Dixon-McCarthy, B., Watkins, S., & Calhoun, W. (2001). Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice. Journal of Leukocyte Biology, 70(5), 730-736.

Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice. / Ameredes, Bill; Zamora, Ruben; Gibson, Kevin F.; Billiar, Timothy R.; Dixon-McCarthy, Barbara; Watkins, Simon; Calhoun, William.

In: Journal of Leukocyte Biology, Vol. 70, No. 5, 2001, p. 730-736.

Research output: Contribution to journalArticle

Ameredes, B, Zamora, R, Gibson, KF, Billiar, TR, Dixon-McCarthy, B, Watkins, S & Calhoun, W 2001, 'Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice', Journal of Leukocyte Biology, vol. 70, no. 5, pp. 730-736.
Ameredes B, Zamora R, Gibson KF, Billiar TR, Dixon-McCarthy B, Watkins S et al. Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice. Journal of Leukocyte Biology. 2001;70(5):730-736.
Ameredes, Bill ; Zamora, Ruben ; Gibson, Kevin F. ; Billiar, Timothy R. ; Dixon-McCarthy, Barbara ; Watkins, Simon ; Calhoun, William. / Increased nitric oxide production by airway cells of sensitized and challenged IL-10 knockout mice. In: Journal of Leukocyte Biology. 2001 ; Vol. 70, No. 5. pp. 730-736.
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