Abstract
We evaluated regional blood flows in a hyperdynamic sepsis model and the reversal of increased flows by blockade of nitric oxide (NO) synthase. Seven awake sheep were continuously infused with Escherichia coli endotoxin [lipopolysaccharide (LPS), 10 ng · kg-1 · min-1] for 48 h. The NO synthase inhibitor N(ω)-nitro-L-arginine methyl ester (L-NAME, 25 mg/kg) was injected after 24 h. Blood flows to systemic organs were determined with the radioactive microsphere technique. LPS induced elevation of cardiac index by 36% (P < 0.05) and a fall in systemic vascular resistance index by 37% (P < 0.05) at 0 h [time of L-NAME administration, 24 h after infusion of LPS had begun]. L-NAME administration normalized cardiac index [6.1 ± 0.5 at 4 h posttreatment, 6.1 ± 0.51 · min-1 · m-2 at -24 h (baseline)] and systemic vascular resistance index (1,333 ± 105 at 4 h posttreatment, 1,280 ± 163 dyn · s · cm-5 · m2 at -24 h) and reduced all regional blood flows to near-baseline levels for the remainder of the study period (24 h). O2 consumption was unaffected by treatment.
Original language | English (US) |
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Pages (from-to) | 2785-2793 |
Number of pages | 9 |
Journal | Journal of Applied Physiology |
Volume | 76 |
Issue number | 6 |
DOIs | |
State | Published - 1994 |
Externally published | Yes |
Keywords
- acute lung injury
- cardiopulmonary
- endotoxin
- lung
- nitric oxide
- oxygen consumption
- pulmonary
- pulmonary hemodynamics
- radioactive microsphere technique
- regional blood flow
- sepsis
ASJC Scopus subject areas
- General Medicine