Increased production of mitochondrial superoxide in the spinal cord induces pain behaviors in mice

The effect of mitochondrial electron transport complex inhibitors

Hee Young Kim, Jin Chung, Kyungsoon Chung

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Scavengers of reactive oxygen species (ROS) have been shown to produce a strong antinociceptive effect on persistent pain, and mitochondria are suggested to be the main source of ROS in the spinal dorsal horn. To explore whether excessive generation of mitochondrial superoxide alone can induce pain, the effect of mitochondrial electron transport complex inhibitors on the development of mechanical hyperalgesia was examined in mice. Intrathecal injection of an electron transport complex inhibitor, antimycin A or rotenone, in normal mice resulted in a slowly developing but long-lasting and dose-dependent mechanical hyperalgesia. The levels of mechanical hyperalgesia after antimycin A, a complex III inhibitor, were higher than that with rotenone, a complex I inhibitor. A large increase of mitochondrial superoxide in the spinal dorsal horn and a strong antinociceptive effect of ROS scavengers, phenyl-N-tert-butylnitrone (PBN) and 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL) were observed in antimycin A-treated mice. The study indicates that the enhanced production of spinal mitochondrial superoxide alone without nerve injury can produce mechanical hyperalgesia.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalNeuroscience Letters
Volume447
Issue number1
DOIs
StatePublished - Dec 5 2008

Fingerprint

Hyperalgesia
Electron Transport
Antimycin A
Superoxides
Spinal Cord
Rotenone
Pain
Reactive Oxygen Species
Spinal Injections
Electron Transport Complex III
Mitochondria
Wounds and Injuries
Spinal Cord Dorsal Horn

Keywords

  • Antimycin A
  • Mechanical hyperalgesia
  • Mitochondrial ETC
  • ROS
  • Rotenone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Increased production of mitochondrial superoxide in the spinal cord induces pain behaviors in mice : The effect of mitochondrial electron transport complex inhibitors. / Kim, Hee Young; Chung, Jin; Chung, Kyungsoon.

In: Neuroscience Letters, Vol. 447, No. 1, 05.12.2008, p. 87-91.

Research output: Contribution to journalArticle

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