Increased uptake of L-cysteine and L-cystine by nerve growth factor in rat pheochromocytoma cells

Zhaohui Pan, Regino Perez-Polo

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Nerve growth factor is a neurotrophic factor which promotes cell survival and differentiation in the central and peripheral nervous system. The rat pheochromocytoma (PC12) cell has been frequently used to study the actions of nerve growth factor (NGF). Our previous studies demonstrate that pretreatment with NGF for 24 h protects PC12 cells from oxidative stress by increasing glutathione (GSH) concentrations and the activity of γ-glutamylcysteine synthetase, which is a rate-limiting enzyme in GSH synthesis. The synthesis of intracellular GSH is dependent on the availability of the precursor amino acid, L-cysteine. Cells take up L-cystine from the extracellular fluid and convert it to L-cysteine intracellularly. L-Cysteine is then released from cells to maintain extracellular L-cysteine. Here we report that NGF increased the uptake of L-cysteine or L-cystine. The increased concentrations of L-cysteine or L-cystine by NGF was responsible for the enhanced intracellular GSH concentrations. The increased GSH and L-cysteine concentrations by NGF also served as intracellular antioxidants. The protection of PC12 cells by NGF from oxidative stress was due to the stimulated increased levels of intracellular glutathione and L-cysteine or L-cystine.

    Original languageEnglish (US)
    Pages (from-to)21-26
    Number of pages6
    JournalBrain Research
    Volume740
    Issue number1-2
    DOIs
    StatePublished - Nov 18 1996

    Fingerprint

    Cystine
    Pheochromocytoma
    Nerve Growth Factor
    Cysteine
    PC12 Cells
    Glutathione
    Oxidative Stress
    Glutamate-Cysteine Ligase
    Extracellular Fluid
    Peripheral Nervous System
    Nerve Growth Factors
    Cell Differentiation
    Cell Survival
    Central Nervous System
    Antioxidants
    Amino Acids
    Enzymes

    Keywords

    • L-cysteine
    • L-cystine
    • nerve growth factor
    • PC12

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Increased uptake of L-cysteine and L-cystine by nerve growth factor in rat pheochromocytoma cells. / Pan, Zhaohui; Perez-Polo, Regino.

    In: Brain Research, Vol. 740, No. 1-2, 18.11.1996, p. 21-26.

    Research output: Contribution to journalArticle

    Pan, Zhaohui ; Perez-Polo, Regino. / Increased uptake of L-cysteine and L-cystine by nerve growth factor in rat pheochromocytoma cells. In: Brain Research. 1996 ; Vol. 740, No. 1-2. pp. 21-26.
    @article{d1af1fc71e6549d882a8f3d8b21cfe57,
    title = "Increased uptake of L-cysteine and L-cystine by nerve growth factor in rat pheochromocytoma cells",
    abstract = "Nerve growth factor is a neurotrophic factor which promotes cell survival and differentiation in the central and peripheral nervous system. The rat pheochromocytoma (PC12) cell has been frequently used to study the actions of nerve growth factor (NGF). Our previous studies demonstrate that pretreatment with NGF for 24 h protects PC12 cells from oxidative stress by increasing glutathione (GSH) concentrations and the activity of γ-glutamylcysteine synthetase, which is a rate-limiting enzyme in GSH synthesis. The synthesis of intracellular GSH is dependent on the availability of the precursor amino acid, L-cysteine. Cells take up L-cystine from the extracellular fluid and convert it to L-cysteine intracellularly. L-Cysteine is then released from cells to maintain extracellular L-cysteine. Here we report that NGF increased the uptake of L-cysteine or L-cystine. The increased concentrations of L-cysteine or L-cystine by NGF was responsible for the enhanced intracellular GSH concentrations. The increased GSH and L-cysteine concentrations by NGF also served as intracellular antioxidants. The protection of PC12 cells by NGF from oxidative stress was due to the stimulated increased levels of intracellular glutathione and L-cysteine or L-cystine.",
    keywords = "L-cysteine, L-cystine, nerve growth factor, PC12",
    author = "Zhaohui Pan and Regino Perez-Polo",
    year = "1996",
    month = "11",
    day = "18",
    doi = "10.1016/S0006-8993(96)00844-X",
    language = "English (US)",
    volume = "740",
    pages = "21--26",
    journal = "Brain Research",
    issn = "0006-8993",
    publisher = "Elsevier",
    number = "1-2",

    }

    TY - JOUR

    T1 - Increased uptake of L-cysteine and L-cystine by nerve growth factor in rat pheochromocytoma cells

    AU - Pan, Zhaohui

    AU - Perez-Polo, Regino

    PY - 1996/11/18

    Y1 - 1996/11/18

    N2 - Nerve growth factor is a neurotrophic factor which promotes cell survival and differentiation in the central and peripheral nervous system. The rat pheochromocytoma (PC12) cell has been frequently used to study the actions of nerve growth factor (NGF). Our previous studies demonstrate that pretreatment with NGF for 24 h protects PC12 cells from oxidative stress by increasing glutathione (GSH) concentrations and the activity of γ-glutamylcysteine synthetase, which is a rate-limiting enzyme in GSH synthesis. The synthesis of intracellular GSH is dependent on the availability of the precursor amino acid, L-cysteine. Cells take up L-cystine from the extracellular fluid and convert it to L-cysteine intracellularly. L-Cysteine is then released from cells to maintain extracellular L-cysteine. Here we report that NGF increased the uptake of L-cysteine or L-cystine. The increased concentrations of L-cysteine or L-cystine by NGF was responsible for the enhanced intracellular GSH concentrations. The increased GSH and L-cysteine concentrations by NGF also served as intracellular antioxidants. The protection of PC12 cells by NGF from oxidative stress was due to the stimulated increased levels of intracellular glutathione and L-cysteine or L-cystine.

    AB - Nerve growth factor is a neurotrophic factor which promotes cell survival and differentiation in the central and peripheral nervous system. The rat pheochromocytoma (PC12) cell has been frequently used to study the actions of nerve growth factor (NGF). Our previous studies demonstrate that pretreatment with NGF for 24 h protects PC12 cells from oxidative stress by increasing glutathione (GSH) concentrations and the activity of γ-glutamylcysteine synthetase, which is a rate-limiting enzyme in GSH synthesis. The synthesis of intracellular GSH is dependent on the availability of the precursor amino acid, L-cysteine. Cells take up L-cystine from the extracellular fluid and convert it to L-cysteine intracellularly. L-Cysteine is then released from cells to maintain extracellular L-cysteine. Here we report that NGF increased the uptake of L-cysteine or L-cystine. The increased concentrations of L-cysteine or L-cystine by NGF was responsible for the enhanced intracellular GSH concentrations. The increased GSH and L-cysteine concentrations by NGF also served as intracellular antioxidants. The protection of PC12 cells by NGF from oxidative stress was due to the stimulated increased levels of intracellular glutathione and L-cysteine or L-cystine.

    KW - L-cysteine

    KW - L-cystine

    KW - nerve growth factor

    KW - PC12

    UR - http://www.scopus.com/inward/record.url?scp=0030592834&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0030592834&partnerID=8YFLogxK

    U2 - 10.1016/S0006-8993(96)00844-X

    DO - 10.1016/S0006-8993(96)00844-X

    M3 - Article

    C2 - 8973794

    AN - SCOPUS:0030592834

    VL - 740

    SP - 21

    EP - 26

    JO - Brain Research

    JF - Brain Research

    SN - 0006-8993

    IS - 1-2

    ER -