Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men

Bart B L Groen, Astrid M H Horstman, Henrike M. Hamer, Michiel De Haan, Janneau Van Kranenburg, Jörgen Bierau, Martijn Poeze, Will K W H Wodzig, Blake Rasmussen, Luc J C Van Loon

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Context: Skeletal muscle protein synthesis is highly responsive to food intake. It has been suggested that the postprandial increase in circulating insulin modulates the muscle protein synthetic response to feeding. Objective: The objective of the study was to investigate whether a greater postprandial rise in circulating insulin level increases amino acid uptake in muscle and augments postprandial muscle protein synthesis rates. Participants and Design: Forty-eight healthy young (age 22±1 y; body mass index 22.0±0.3 kg/m2) and older males (age 68 ± 1 y; body mass index 26.3 ± 0.4 kg/m2) ingested 20 g intrinsically L-[1-13C]-leucine-and L-[1-13C]-phenylalanine-labeled casein protein with or without local insulin infusion. Primed continuous infusions of L-[1-13C]-leucine and L-[ring-2H5]-phenylalanine were applied, with arterial and venous blood samples and muscle biopsies being collected during a 5-hour postprandial period. Results: Insulin administration did not increase overall leg blood flow (P = .509) but increased amino acid uptake over the leg in both young and older subjects (P = .003). The greater amino acid uptake over the leg did not further increase postprandial muscle protein synthesis rates (0.050% ± 0.006% and 0.037% ± 0.004% per hour vs 0.044% ± 0.004% and 0.037% ± 0.002% per hour in the insulin-stimulated vs control condition in the young and older groups, respectively; P = .804) and did not affect postprandial deposition of dietary protein-derived amino acids in de novo muscle protein (P = .872). Conclusion: Greater postprandial plasma insulin availability stimulates amino acid uptake over the leg but does not further augment postprandial muscle protein synthesis rates or stimulate the postprandial deposition of protein derived amino acids into de novo muscle protein in healthy young and older men. (J Clin Endocrinol Metab 101: 3978-3988, 2016).

    Original languageEnglish (US)
    Pages (from-to)3978-3988
    Number of pages11
    JournalJournal of Clinical Endocrinology and Metabolism
    Volume101
    Issue number11
    DOIs
    StatePublished - Nov 1 2016

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    Muscle Proteins
    Availability
    Insulin
    Amino Acids
    Leg
    Phenylalanine
    Leucine
    Muscle
    Body Mass Index
    Blood
    Postprandial Period
    Muscles
    Dietary Proteins
    Biopsy
    Caseins
    Skeletal Muscle
    Proteins
    Eating
    Plasmas

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Biochemistry
    • Endocrinology
    • Clinical Biochemistry
    • Biochemistry, medical

    Cite this

    Groen, B. B. L., Horstman, A. M. H., Hamer, H. M., De Haan, M., Van Kranenburg, J., Bierau, J., ... Van Loon, L. J. C. (2016). Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men. Journal of Clinical Endocrinology and Metabolism, 101(11), 3978-3988. https://doi.org/10.1210/jc.2016-1436

    Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men. / Groen, Bart B L; Horstman, Astrid M H; Hamer, Henrike M.; De Haan, Michiel; Van Kranenburg, Janneau; Bierau, Jörgen; Poeze, Martijn; Wodzig, Will K W H; Rasmussen, Blake; Van Loon, Luc J C.

    In: Journal of Clinical Endocrinology and Metabolism, Vol. 101, No. 11, 01.11.2016, p. 3978-3988.

    Research output: Contribution to journalArticle

    Groen, BBL, Horstman, AMH, Hamer, HM, De Haan, M, Van Kranenburg, J, Bierau, J, Poeze, M, Wodzig, WKWH, Rasmussen, B & Van Loon, LJC 2016, 'Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men', Journal of Clinical Endocrinology and Metabolism, vol. 101, no. 11, pp. 3978-3988. https://doi.org/10.1210/jc.2016-1436
    Groen, Bart B L ; Horstman, Astrid M H ; Hamer, Henrike M. ; De Haan, Michiel ; Van Kranenburg, Janneau ; Bierau, Jörgen ; Poeze, Martijn ; Wodzig, Will K W H ; Rasmussen, Blake ; Van Loon, Luc J C. / Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men. In: Journal of Clinical Endocrinology and Metabolism. 2016 ; Vol. 101, No. 11. pp. 3978-3988.
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    abstract = "Context: Skeletal muscle protein synthesis is highly responsive to food intake. It has been suggested that the postprandial increase in circulating insulin modulates the muscle protein synthetic response to feeding. Objective: The objective of the study was to investigate whether a greater postprandial rise in circulating insulin level increases amino acid uptake in muscle and augments postprandial muscle protein synthesis rates. Participants and Design: Forty-eight healthy young (age 22±1 y; body mass index 22.0±0.3 kg/m2) and older males (age 68 ± 1 y; body mass index 26.3 ± 0.4 kg/m2) ingested 20 g intrinsically L-[1-13C]-leucine-and L-[1-13C]-phenylalanine-labeled casein protein with or without local insulin infusion. Primed continuous infusions of L-[1-13C]-leucine and L-[ring-2H5]-phenylalanine were applied, with arterial and venous blood samples and muscle biopsies being collected during a 5-hour postprandial period. Results: Insulin administration did not increase overall leg blood flow (P = .509) but increased amino acid uptake over the leg in both young and older subjects (P = .003). The greater amino acid uptake over the leg did not further increase postprandial muscle protein synthesis rates (0.050{\%} ± 0.006{\%} and 0.037{\%} ± 0.004{\%} per hour vs 0.044{\%} ± 0.004{\%} and 0.037{\%} ± 0.002{\%} per hour in the insulin-stimulated vs control condition in the young and older groups, respectively; P = .804) and did not affect postprandial deposition of dietary protein-derived amino acids in de novo muscle protein (P = .872). Conclusion: Greater postprandial plasma insulin availability stimulates amino acid uptake over the leg but does not further augment postprandial muscle protein synthesis rates or stimulate the postprandial deposition of protein derived amino acids into de novo muscle protein in healthy young and older men. (J Clin Endocrinol Metab 101: 3978-3988, 2016).",
    author = "Groen, {Bart B L} and Horstman, {Astrid M H} and Hamer, {Henrike M.} and {De Haan}, Michiel and {Van Kranenburg}, Janneau and J{\"o}rgen Bierau and Martijn Poeze and Wodzig, {Will K W H} and Blake Rasmussen and {Van Loon}, {Luc J C}",
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    T1 - Increasing insulin availability does not augment postprandial muscle protein synthesis rates in healthy young and older men

    AU - Groen, Bart B L

    AU - Horstman, Astrid M H

    AU - Hamer, Henrike M.

    AU - De Haan, Michiel

    AU - Van Kranenburg, Janneau

    AU - Bierau, Jörgen

    AU - Poeze, Martijn

    AU - Wodzig, Will K W H

    AU - Rasmussen, Blake

    AU - Van Loon, Luc J C

    PY - 2016/11/1

    Y1 - 2016/11/1

    N2 - Context: Skeletal muscle protein synthesis is highly responsive to food intake. It has been suggested that the postprandial increase in circulating insulin modulates the muscle protein synthetic response to feeding. Objective: The objective of the study was to investigate whether a greater postprandial rise in circulating insulin level increases amino acid uptake in muscle and augments postprandial muscle protein synthesis rates. Participants and Design: Forty-eight healthy young (age 22±1 y; body mass index 22.0±0.3 kg/m2) and older males (age 68 ± 1 y; body mass index 26.3 ± 0.4 kg/m2) ingested 20 g intrinsically L-[1-13C]-leucine-and L-[1-13C]-phenylalanine-labeled casein protein with or without local insulin infusion. Primed continuous infusions of L-[1-13C]-leucine and L-[ring-2H5]-phenylalanine were applied, with arterial and venous blood samples and muscle biopsies being collected during a 5-hour postprandial period. Results: Insulin administration did not increase overall leg blood flow (P = .509) but increased amino acid uptake over the leg in both young and older subjects (P = .003). The greater amino acid uptake over the leg did not further increase postprandial muscle protein synthesis rates (0.050% ± 0.006% and 0.037% ± 0.004% per hour vs 0.044% ± 0.004% and 0.037% ± 0.002% per hour in the insulin-stimulated vs control condition in the young and older groups, respectively; P = .804) and did not affect postprandial deposition of dietary protein-derived amino acids in de novo muscle protein (P = .872). Conclusion: Greater postprandial plasma insulin availability stimulates amino acid uptake over the leg but does not further augment postprandial muscle protein synthesis rates or stimulate the postprandial deposition of protein derived amino acids into de novo muscle protein in healthy young and older men. (J Clin Endocrinol Metab 101: 3978-3988, 2016).

    AB - Context: Skeletal muscle protein synthesis is highly responsive to food intake. It has been suggested that the postprandial increase in circulating insulin modulates the muscle protein synthetic response to feeding. Objective: The objective of the study was to investigate whether a greater postprandial rise in circulating insulin level increases amino acid uptake in muscle and augments postprandial muscle protein synthesis rates. Participants and Design: Forty-eight healthy young (age 22±1 y; body mass index 22.0±0.3 kg/m2) and older males (age 68 ± 1 y; body mass index 26.3 ± 0.4 kg/m2) ingested 20 g intrinsically L-[1-13C]-leucine-and L-[1-13C]-phenylalanine-labeled casein protein with or without local insulin infusion. Primed continuous infusions of L-[1-13C]-leucine and L-[ring-2H5]-phenylalanine were applied, with arterial and venous blood samples and muscle biopsies being collected during a 5-hour postprandial period. Results: Insulin administration did not increase overall leg blood flow (P = .509) but increased amino acid uptake over the leg in both young and older subjects (P = .003). The greater amino acid uptake over the leg did not further increase postprandial muscle protein synthesis rates (0.050% ± 0.006% and 0.037% ± 0.004% per hour vs 0.044% ± 0.004% and 0.037% ± 0.002% per hour in the insulin-stimulated vs control condition in the young and older groups, respectively; P = .804) and did not affect postprandial deposition of dietary protein-derived amino acids in de novo muscle protein (P = .872). Conclusion: Greater postprandial plasma insulin availability stimulates amino acid uptake over the leg but does not further augment postprandial muscle protein synthesis rates or stimulate the postprandial deposition of protein derived amino acids into de novo muscle protein in healthy young and older men. (J Clin Endocrinol Metab 101: 3978-3988, 2016).

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