Indole-3-propionate: A potent hydroxyl radical scavenger in rat brain

Burkhard Poeggeler, Miguel A. Pappolla, Rüdiger Hardeland, Arash Rassoulpour, Paul S. Hodgkins, Paolo Guidetti, Robert Schwarcz

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The hydroxyl radical scavenging activity of indole-3-propionate was evaluated by kinetic competition studies with the hydroxyl radical trapping reagent 2,2'-azino-bis-(3-ethyl-benz-thiazoline-6-sulfonic acid) (ABTS) and by measuring hydroxyl radical-initiated lipid peroxidation in the rat striatum. Using ABTS, the indole was shown to act as a potent hydroxyl radical scavenger with a rate constant of 7.8 X 1010 mol l-1 s-1. Hydroxyl radical-initiated lipid peroxidation, determined by measuring tissue malondialdehyde formation, was inhibited dose-dependently both in vitro and in vivo. Indole-3-propionate reacts with hydroxyl radicals at a diffusion controlled rate and can thereby provide on-site protection against the oxidative damage of biomolecules induced by these highly reactive and toxic oxygen intermediates. While it remains to be established if endogenous brain tissue levels of indole-3-propionate are sufficiently high to have a significant impact on total antioxidative capacity, the compound itself or a structurally related agent may be useful as an antioxidant adjuvant to combat hydroxyl radical-mediated oxidative stress.

Original languageEnglish (US)
Pages (from-to)382-388
Number of pages7
JournalBrain Research
Volume815
Issue number2
DOIs
StatePublished - Jan 9 1999
Externally publishedYes

Keywords

  • Glutathione
  • Indole acids
  • Lipid peroxidation
  • Neurodegeneration
  • Neuroprotection
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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