Indomethacin inhibits cholera toxin-induced cyclic AMP accumulation in Chinese hamster ovary cells

Johnny W. Peterson, William D. Berg, Laura G. Ochoa

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Indomethacin was examined for its capacity to inhibit increases in adenosine-3′,5′-monophosphate (cAMP) concentrations in Chinese hamster ovary (CHO) cells treated with cholera toxin. When added to the culture medium 1 h prior to cholera toxin (100 ng/ml), indomethacin (500 μg/ml) exhibited maximum protection against the typical increase in cAMP. Application of indomethacin at the same time as cholera toxin or up to 3 h after the toxin progressively decreased the drug's capacity to block further increases in cAMP. The drug appeared to block adenylate cyclase activity because addition of forskolin to drug-treated cells did not elicit a cAMP response. Binding of 125I-labeled cholera toxin to indomethacin-treated cells was also reduced by at least 50%. These data indicate that indomethacin's inhibitory effect on cAMP formation in cholera toxin-treated cells could be explained by its capacity to alter adenylate cyclase activity and cholera toxin binding.

Original languageEnglish (US)
Pages (from-to)187-192
Number of pages6
JournalFEMS Microbiology Letters
Volume49
Issue number2
DOIs
StatePublished - Feb 1988

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Keywords

  • Chinese hamster ovary
  • Cholera toxin
  • Cyclic AMP
  • Indomethacin

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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