Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour

Tracy L. Purcell, Irina A. Buhimschi, Randall Given, Kristof Chwalisz, Robert E. Garfield

    Research output: Contribution to journalArticle

    62 Citations (Scopus)

    Abstract

    The aim of this study was to investigate the expression and distribution patterns of the inducible isoform of nitric oxide synthase (iNOS) in rat placenta during gestation and term labour. The expression of iNOS isoform was assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting with monoclonal antibodies. Two specific bands were detected corresponding to 135 and 124 kDa in all placenta samples. The upper band (135 kDa) was identified as iNOS due to its correspondence with the band obtained with mouse macrophages (positive control). Compared with its concentrations on day 16, iNOS decreased steadily toward the end of gestation to ∼37% on day 20, 20% on day 22 before labour and 12% during labour (P <0.01). The lower band (124 kDa) drastically increased (to almost double) from day 16 to day 18 but returned to initial values on day 22, during delivery. Immunohistochemical staining of placentae at day 16 and 22 using rabbit polyclonal anti-iNOS antibody revealed labelling specifically concentrated in the trophospongial cell layer, at the fetal-maternal interface. The most conspicuous iNOS staining was associated with islands of cells referred to as vacuolated 'glycogen cells'. Staining was greatly decreased during labour. The changes in placental iNOS expression suggest a 'paracrine' role for NO in regulating uterine contractility, blood flow and immunosuppression required for pregnancy maintenance, NO withdrawal at term may also be involved in the initiation of labour.

    Original languageEnglish (US)
    Pages (from-to)485-491
    Number of pages7
    JournalMolecular Human Reproduction
    Volume3
    Issue number6
    StatePublished - 1997

    Fingerprint

    Nitric Oxide Synthase Type II
    Placenta
    Protein Isoforms
    Mothers
    Nitric Oxide Synthase
    Staining and Labeling
    Pregnancy Maintenance
    Pregnancy
    Glycogen
    Immunoblotting
    Sodium Dodecyl Sulfate
    Immunosuppression
    Polyacrylamide Gel Electrophoresis
    Macrophages
    Monoclonal Antibodies
    Rabbits
    Antibodies

    Keywords

    • Immunohistochemistry
    • Labour
    • Nitric oxide
    • Placenta
    • Western blot

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Genetics
    • Embryology
    • Obstetrics and Gynecology

    Cite this

    Purcell, T. L., Buhimschi, I. A., Given, R., Chwalisz, K., & Garfield, R. E. (1997). Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour. Molecular Human Reproduction, 3(6), 485-491.

    Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour. / Purcell, Tracy L.; Buhimschi, Irina A.; Given, Randall; Chwalisz, Kristof; Garfield, Robert E.

    In: Molecular Human Reproduction, Vol. 3, No. 6, 1997, p. 485-491.

    Research output: Contribution to journalArticle

    Purcell, TL, Buhimschi, IA, Given, R, Chwalisz, K & Garfield, RE 1997, 'Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour', Molecular Human Reproduction, vol. 3, no. 6, pp. 485-491.
    Purcell, Tracy L. ; Buhimschi, Irina A. ; Given, Randall ; Chwalisz, Kristof ; Garfield, Robert E. / Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour. In: Molecular Human Reproduction. 1997 ; Vol. 3, No. 6. pp. 485-491.
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