Induction of a subgroup of acute phase protein genes in mouse liver by hyperthermia

Minas Yiangou, Efrosini Paraskeva, Ching Chyuan Hsieh, Eustathia Markou, Panayiotis Victoratos, Zacharias Scouras, John Papaconstantinou

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We have demonstrated that two members of the acute phase reactant family of positively regulated genes, α1-acid glycoprotein (AGP-1 and AGP-2) and C-reactive protein (CRP) are induced by hyperthermia, while two others, the serum amyloid A (SAA) and α1-antitrypsin (AT) genes, are not. Albumin (ALB), a negative acute phase reactant gene, is also induced by hyperthermia. The AGP-1, AGP-2, and CRP genes require glucocorticoids, but not IL-6, IL- 1β or TNFα in response to hyperthermia. As with LPS, the C/EBPβ mRNA levels increased, while the C/EBPα mRNA levels decreased in response to LPS. In contrast to the LPS response, C/EBPδ was unchanged. Protein pool levels and DNA-binding activities of the 35 and 20 kDa C/EBPβ isoforms increase, whereas protein pool levels of the 42 kDa C/EBPα decrease and the 30 kDa remained high. These studies suggest that the synthesis of specific C/EBPα and C/EBPβ isoforms is induced by hyperthermia, and that the regulation of the AGP-1 and AGP-2 genes during heat stress may involve one of these isoforms. The difference between the responses to hyperthermia and LPS is that the former, may not involve the participation of cytokines. Furthermore, since cis-acting heat shock elements (HSE) are located in the promoter regions of the ALB, CRP, and C/EBPβ genes, these regulatory sequences may be involved in the in vivo activation of these genes by hyperthermia.

Original languageEnglish (US)
Pages (from-to)191-206
Number of pages16
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1396
Issue number2
DOIs
StatePublished - Mar 9 1998

Fingerprint

Acute-Phase Proteins
Liver
Fever
Genes
Induced Hyperthermia
C-Reactive Protein
Protein Isoforms
Albumins
Hot Temperature
Serum Amyloid A Protein
Messenger RNA
Regulator Genes
Protein C
Interleukin-1
Genetic Promoter Regions
Transcriptional Activation
Glucocorticoids
Shock
Interleukin-6
Glycoproteins

Keywords

  • α-Acid glycoprotein
  • Acute phase response
  • C-reactive protein
  • C/EBP
  • Heat shock

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Induction of a subgroup of acute phase protein genes in mouse liver by hyperthermia. / Yiangou, Minas; Paraskeva, Efrosini; Hsieh, Ching Chyuan; Markou, Eustathia; Victoratos, Panayiotis; Scouras, Zacharias; Papaconstantinou, John.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1396, No. 2, 09.03.1998, p. 191-206.

Research output: Contribution to journalArticle

Yiangou, Minas ; Paraskeva, Efrosini ; Hsieh, Ching Chyuan ; Markou, Eustathia ; Victoratos, Panayiotis ; Scouras, Zacharias ; Papaconstantinou, John. / Induction of a subgroup of acute phase protein genes in mouse liver by hyperthermia. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1998 ; Vol. 1396, No. 2. pp. 191-206.
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