Induction of apoptosis in human gastric cancer by sodium butyrate

David A. Litvak, Kevin O. Hwang, B. Mark Evers, Courtney Townsend

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background. Novel therapeutic agents are needed in the adjuvant treatment of gastric cancer. The differentiating agent sodium butyrate (NaBT) inhibits the growth of colon cancer cells; its effects on gastric cancers are not known. The purpose of our study was to characterize the effects of NaBT on human gastric cancer. Material and Methods. The human gastric cancer, SIIA, was treated with NaBT (5 mM) for 12-72 h. Cell number, viability and death were measured. Expression levels of the tumor-suppressor protein, p53, the cell-cycle inhibitors, p21(Waf1/Cip1) and p27(Kip1), and the pro-apoptotic proteins, Bax, Bak, and Bik, were determined. Results. NaBT significantly inhibited SILA gastric cancer cell proliferation in a time-dependent fashion by a process involving the induction of apoptosis. Treatment with NaBT was associated with increased expression levels of p21(Waf1/Cip1) p27(Kip1), Bax, Bak, and Bik. Conclusions. NaBT triggers growth arrest and apoptosis in the human gastric cancer SIIA potentially through the induction of the cell-cycle inhibitors, p21(Waf1/Cip1) and p27(Kip1), and the pro-apoptotic genes, Bax, Bak, and Bik. NaBT may be an effective adjuvant agent in the treatment of gastric cancer.

Original languageEnglish (US)
Pages (from-to)779-784
Number of pages6
JournalAnticancer Research
Volume20
Issue number2 A
StatePublished - 2000

Fingerprint

Butyric Acid
Stomach Neoplasms
Apoptosis
Cell Cycle
Tumor Suppressor Protein p53
Apoptosis Regulatory Proteins
Growth
Colonic Neoplasms
Cell Survival
Cell Death
Cell Count
Cell Proliferation
Genes

Keywords

  • Apoptosis
  • Cell cycle
  • Gastric cancer
  • Sodium butyrate

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Litvak, D. A., Hwang, K. O., Evers, B. M., & Townsend, C. (2000). Induction of apoptosis in human gastric cancer by sodium butyrate. Anticancer Research, 20(2 A), 779-784.

Induction of apoptosis in human gastric cancer by sodium butyrate. / Litvak, David A.; Hwang, Kevin O.; Evers, B. Mark; Townsend, Courtney.

In: Anticancer Research, Vol. 20, No. 2 A, 2000, p. 779-784.

Research output: Contribution to journalArticle

Litvak, DA, Hwang, KO, Evers, BM & Townsend, C 2000, 'Induction of apoptosis in human gastric cancer by sodium butyrate', Anticancer Research, vol. 20, no. 2 A, pp. 779-784.
Litvak, David A. ; Hwang, Kevin O. ; Evers, B. Mark ; Townsend, Courtney. / Induction of apoptosis in human gastric cancer by sodium butyrate. In: Anticancer Research. 2000 ; Vol. 20, No. 2 A. pp. 779-784.
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