Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer

Sungwhan An, Chun Jen Chen, Xin Yu, Julian L. Leibowitz, Shinji Makino

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

We demonstrated that infection of 17Cl-1 cells with the murine coronavirus mouse hepatitis virus (MHV) induced caspase-dependent apoptosis. MHV-infected DBT cells did not show apoptotic changes, indicating that apoptosis was not a universal mechanism of cell death in MHV-infected cells. Expression of MHV structural proteins by recombinant vaccinia viruses showed that expression of MHV E protein induced apoptosis in DBT cells, whereas expression of other MHV structural proteins, including S protein, M protein, N protein, and hemagglutinin-esterase protein, failed to induce apoptosis. MHV E protein-mediated apoptosis was suppressed by a high level of Bcl-2 oncogene expression. Our data showed that MHV E protein is a multifunctional protein; in addition to its known function in coronavirus envelope formation, it also induces apoptosis.

Original languageEnglish (US)
Pages (from-to)7853-7859
Number of pages7
JournalJournal of virology
Volume73
Issue number9
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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