Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer

Sungwhan An, Chun Jen Chen, Xin Yu, Julian L. Leibowitz, Shinji Makino

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Abstract

We demonstrated that infection of 17Cl-1 cells with the murine coronavirus mouse hepatitis virus (MHV) induced caspase-dependent apoptosis. MHV-infected DBT cells did not show apoptotic changes, indicating that apoptosis was not a universal mechanism of cell death in MHV-infected cells. Expression of MHV structural proteins by recombinant vaccinia viruses showed that expression of MHV E protein induced apoptosis in DBT cells, whereas expression of other MHV structural proteins, including S protein, M protein, N protein, and hemagglutinin-esterase protein, failed to induce apoptosis. MHV E protein-mediated apoptosis was suppressed by a high level of Bcl-2 oncogene expression. Our data showed that MHV E protein is a multifunctional protein; in addition to its known function in coronavirus envelope formation, it also induces apoptosis.

Original languageEnglish (US)
Pages (from-to)7853-7859
Number of pages7
JournalJournal of Virology
Volume73
Issue number9
StatePublished - 1999

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Murine coronavirus
Murine hepatitis virus
Coronavirus
cultured cells
Cultured Cells
apoptosis
Apoptosis
Hepatitis E
Proteins
proteins
Viral Structural Proteins
structural proteins
cells
Vaccinia virus
Coronavirinae
oncogenes
Protein S
caspases
hemagglutinins
Caspases

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer. / An, Sungwhan; Chen, Chun Jen; Yu, Xin; Leibowitz, Julian L.; Makino, Shinji.

In: Journal of Virology, Vol. 73, No. 9, 1999, p. 7853-7859.

Research output: Contribution to journalArticle

An, Sungwhan ; Chen, Chun Jen ; Yu, Xin ; Leibowitz, Julian L. ; Makino, Shinji. / Induction of apoptosis in murine coronavirus-infected cultured cells and demonstration of E protein as an apoptosis inducer. In: Journal of Virology. 1999 ; Vol. 73, No. 9. pp. 7853-7859.
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