Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells

Treetip Chiewchanwit, Hongbao Ma, Randa El Zein, Lance Hallberg, William W. Au

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4% which is 2.3-fold higher than that from spontaneous mutants (18.6%). The frequency of ENU-induced deletion mutation is 3%. The data substantiate our hypothesis that MALD induces major deletion mutations.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalMutation Research/Environmental Mutagenesis and Related Subjects
Volume335
Issue number2
DOIs
StatePublished - 1995

Fingerprint

Sequence Deletion
Cricetulus
Ovary
Ethylnitrosourea
Genes
Mutation
Polymerase chain reaction
Gene Deletion
Chromosomes
Metabolites
Aberrations
Point Mutation
Chromosome Aberrations
2-methoxyacetaldehyde
Assays
Cells
Cell Line
Polymerase Chain Reaction

Keywords

  • Chinese hamster ovary cells
  • Deletion mutation
  • Methoxyacetaldehyde
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Genetics
  • Toxicology

Cite this

Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells. / Chiewchanwit, Treetip; Ma, Hongbao; El Zein, Randa; Hallberg, Lance; Au, William W.

In: Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 335, No. 2, 1995, p. 121-128.

Research output: Contribution to journalArticle

Chiewchanwit, Treetip ; Ma, Hongbao ; El Zein, Randa ; Hallberg, Lance ; Au, William W. / Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells. In: Mutation Research/Environmental Mutagenesis and Related Subjects. 1995 ; Vol. 335, No. 2. pp. 121-128.
@article{d32f3d6c19de4e5cbd1eee029c8cfc29,
title = "Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells",
abstract = "We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4{\%} which is 2.3-fold higher than that from spontaneous mutants (18.6{\%}). The frequency of ENU-induced deletion mutation is 3{\%}. The data substantiate our hypothesis that MALD induces major deletion mutations.",
keywords = "Chinese hamster ovary cells, Deletion mutation, Methoxyacetaldehyde, Polymerase chain reaction",
author = "Treetip Chiewchanwit and Hongbao Ma and {El Zein}, Randa and Lance Hallberg and Au, {William W.}",
year = "1995",
doi = "10.1016/0165-1161(95)90049-7",
language = "English (US)",
volume = "335",
pages = "121--128",
journal = "Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology",
issn = "0165-1161",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells

AU - Chiewchanwit, Treetip

AU - Ma, Hongbao

AU - El Zein, Randa

AU - Hallberg, Lance

AU - Au, William W.

PY - 1995

Y1 - 1995

N2 - We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4% which is 2.3-fold higher than that from spontaneous mutants (18.6%). The frequency of ENU-induced deletion mutation is 3%. The data substantiate our hypothesis that MALD induces major deletion mutations.

AB - We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4% which is 2.3-fold higher than that from spontaneous mutants (18.6%). The frequency of ENU-induced deletion mutation is 3%. The data substantiate our hypothesis that MALD induces major deletion mutations.

KW - Chinese hamster ovary cells

KW - Deletion mutation

KW - Methoxyacetaldehyde

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0028846509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028846509&partnerID=8YFLogxK

U2 - 10.1016/0165-1161(95)90049-7

DO - 10.1016/0165-1161(95)90049-7

M3 - Article

C2 - 7477042

AN - SCOPUS:0028846509

VL - 335

SP - 121

EP - 128

JO - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology

JF - Mutation Research - Environmental Mutagenesis and Related Subjects Including Methodology

SN - 0165-1161

IS - 2

ER -