Induction of deletion mutations by methoxyacetaldehyde in Chinese hamster ovary (CHO)-AS52 cells

Treetip Chiewchanwit, Hongbao Ma, Randa El Zein, Lance Hallberg, William W. Au

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Abstract

We have report previously that methoxyacetaldehyde (MALD), a metabolite of 2-methoxyethanol, induces gpt gene mutations in Chinese hamster ovary (CHO)-AS52 cells but not hprt gene mutations in the standard CHO-K1-BH4 cells. In addition, MALD induces chromosome aberrations in both CHO cell lines. The data presented suggest that MALD induces deletion-type mutations. In this study, we analyzed MALD-induced CHO-AS52 mutants for deletion-type mutations using the nested-polymerase chain reaction (nested-PCR) assay. Spontaneous CHO-AS52 mutants are used as untreated control. Ethylnitrosourea (ENU)-induced CHO-AS52 mutants are used as negative control for multilocus deletions since ENU is a potent inducer of point mutations. The results show that the frequency of MALD-induced mutants containing total deletion of the gpt gene is 42.4% which is 2.3-fold higher than that from spontaneous mutants (18.6%). The frequency of ENU-induced deletion mutation is 3%. The data substantiate our hypothesis that MALD induces major deletion mutations.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalMutation Research/Environmental Mutagenesis and Related Subjects
Volume335
Issue number2
DOIs
StatePublished - Oct 1995

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Keywords

  • Chinese hamster ovary cells
  • Deletion mutation
  • Methoxyacetaldehyde
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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