Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

Kota V. Ramana, Krishan K. Kohli

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalDrug and Chemical Toxicology
Volume19
Issue number1-2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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