Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta)

Kota Ramana, Krishan K. Kohli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An intraperitoneal administration of PB at a daily dose of 50 mg Kg-1 body wt for 4 days increased the specific content of hepatic microsomal heine, cytochrome P450 and the activity of aminopyrine N-demethylase by 1.8, 2.8 and 3.5 fold respectively. These results were substantiated by the intensification of the 52.5 KDa polypeptide in the electrophoretogram of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the hepatic microsomes obtained from PB - pretreated versus control macaques. PB did not affect the hepatic content of cytochrome b5 and the activity of NADPH cytochrome c reductase, whereas it decreased the activity of NADH cytochrome c reductase in the rhesus monkeys. To the best of our knowledge this is a first report on the induction of hepatic cytochrome P450 and related enzymes by PB in rhesus monkeys.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalDrug and Chemical Toxicology
Volume19
Issue number1-2
StatePublished - 1996
Externally publishedYes

Fingerprint

Phenobarbital
Macaca mulatta
Cytochrome P-450 Enzyme System
Aminopyrine N-Demethylase
Proteins
Cytochromes b5
NADH Dehydrogenase
NADPH-Ferrihemoprotein Reductase
Liver
Electrophoresis
Sodium Dodecyl Sulfate
Polypeptides
Sodium dodecyl sulfate
Polyacrylates
Peptides
Macaca
Enzymes
Gels
Microsomes
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Induction of hepatic cytochrome P450 by phenobarbitone in rhesus monkey (Macaca mulatta). / Ramana, Kota; Kohli, Krishan K.

In: Drug and Chemical Toxicology, Vol. 19, No. 1-2, 1996, p. 97-107.

Research output: Contribution to journalArticle

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