Induction of human gamma interferon by structurally defined polypeptide fragments of group A streptococcal M protein

D. A. Weigent, E. H. Beachey, T. Huff, Johnny Peterson, G. J. Stanton, S. Baron

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The presence of interferon (IFN) has been demonstrated previously (i) in fluids obtained from the middle ears of children with Streptococcus pneumoniae infections, (ii) from the serum of mice injected intraperitoneally with either S. pneumoniae or Streptococcus pyogenes, and (iii) from human lymphoid cell cultures treated with a variety of bacteria. In this study, we showed that highly purified peptic extracts of three different serotypes of group A streptococcal M protein (pep M5, pep M6, and pep M24) stimulated human peripheral leukocytes to produce IFN. IFN production was apparent by 10 h and peaked 24 h after exposure. Dose-response experiments indicated that IFN could be detected in cultures treated with concentrations of M protein as low as 6 μg/ml, whereas maximum IFN production occurred at a concentration of 200 μg/ml. The IFN had antigenic and physicochemical characteristics of IFN-γ. Preliminary leukocyte fractionation studies revealed that the IFN-producing cell was a nonadherent lymphocyte with receptors for sheep erythrocytes (T cell). Rabbit antisera specific for these structurally defined polypeptide fragments of streptococcal M protein (pep M5, pep M6, and pep M24) blocked IFN induction by each of the polypeptides. The data suggest that the different serotypes of streptococcal M protein may induce IFN by a common structural determinant shared by each of the polypeptide fragments tested.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalInfection and Immunity
Volume43
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Interferons
Peptides
Leukocytes
streptococcal M protein
human IFNG protein
Lymphocytes
Pneumococcal Infections
Streptococcus pyogenes
Middle Ear
Streptococcus pneumoniae
Immune Sera
Digestion
Sheep
Cell Culture Techniques
Erythrocytes
Rabbits
Bacteria
T-Lymphocytes
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of human gamma interferon by structurally defined polypeptide fragments of group A streptococcal M protein. / Weigent, D. A.; Beachey, E. H.; Huff, T.; Peterson, Johnny; Stanton, G. J.; Baron, S.

In: Infection and Immunity, Vol. 43, No. 1, 1984, p. 122-126.

Research output: Contribution to journalArticle

Weigent, D. A. ; Beachey, E. H. ; Huff, T. ; Peterson, Johnny ; Stanton, G. J. ; Baron, S. / Induction of human gamma interferon by structurally defined polypeptide fragments of group A streptococcal M protein. In: Infection and Immunity. 1984 ; Vol. 43, No. 1. pp. 122-126.
@article{fa037a1d19794e38a5490bec37f33696,
title = "Induction of human gamma interferon by structurally defined polypeptide fragments of group A streptococcal M protein",
abstract = "The presence of interferon (IFN) has been demonstrated previously (i) in fluids obtained from the middle ears of children with Streptococcus pneumoniae infections, (ii) from the serum of mice injected intraperitoneally with either S. pneumoniae or Streptococcus pyogenes, and (iii) from human lymphoid cell cultures treated with a variety of bacteria. In this study, we showed that highly purified peptic extracts of three different serotypes of group A streptococcal M protein (pep M5, pep M6, and pep M24) stimulated human peripheral leukocytes to produce IFN. IFN production was apparent by 10 h and peaked 24 h after exposure. Dose-response experiments indicated that IFN could be detected in cultures treated with concentrations of M protein as low as 6 μg/ml, whereas maximum IFN production occurred at a concentration of 200 μg/ml. The IFN had antigenic and physicochemical characteristics of IFN-γ. Preliminary leukocyte fractionation studies revealed that the IFN-producing cell was a nonadherent lymphocyte with receptors for sheep erythrocytes (T cell). Rabbit antisera specific for these structurally defined polypeptide fragments of streptococcal M protein (pep M5, pep M6, and pep M24) blocked IFN induction by each of the polypeptides. The data suggest that the different serotypes of streptococcal M protein may induce IFN by a common structural determinant shared by each of the polypeptide fragments tested.",
author = "Weigent, {D. A.} and Beachey, {E. H.} and T. Huff and Johnny Peterson and Stanton, {G. J.} and S. Baron",
year = "1984",
language = "English (US)",
volume = "43",
pages = "122--126",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Induction of human gamma interferon by structurally defined polypeptide fragments of group A streptococcal M protein

AU - Weigent, D. A.

AU - Beachey, E. H.

AU - Huff, T.

AU - Peterson, Johnny

AU - Stanton, G. J.

AU - Baron, S.

PY - 1984

Y1 - 1984

N2 - The presence of interferon (IFN) has been demonstrated previously (i) in fluids obtained from the middle ears of children with Streptococcus pneumoniae infections, (ii) from the serum of mice injected intraperitoneally with either S. pneumoniae or Streptococcus pyogenes, and (iii) from human lymphoid cell cultures treated with a variety of bacteria. In this study, we showed that highly purified peptic extracts of three different serotypes of group A streptococcal M protein (pep M5, pep M6, and pep M24) stimulated human peripheral leukocytes to produce IFN. IFN production was apparent by 10 h and peaked 24 h after exposure. Dose-response experiments indicated that IFN could be detected in cultures treated with concentrations of M protein as low as 6 μg/ml, whereas maximum IFN production occurred at a concentration of 200 μg/ml. The IFN had antigenic and physicochemical characteristics of IFN-γ. Preliminary leukocyte fractionation studies revealed that the IFN-producing cell was a nonadherent lymphocyte with receptors for sheep erythrocytes (T cell). Rabbit antisera specific for these structurally defined polypeptide fragments of streptococcal M protein (pep M5, pep M6, and pep M24) blocked IFN induction by each of the polypeptides. The data suggest that the different serotypes of streptococcal M protein may induce IFN by a common structural determinant shared by each of the polypeptide fragments tested.

AB - The presence of interferon (IFN) has been demonstrated previously (i) in fluids obtained from the middle ears of children with Streptococcus pneumoniae infections, (ii) from the serum of mice injected intraperitoneally with either S. pneumoniae or Streptococcus pyogenes, and (iii) from human lymphoid cell cultures treated with a variety of bacteria. In this study, we showed that highly purified peptic extracts of three different serotypes of group A streptococcal M protein (pep M5, pep M6, and pep M24) stimulated human peripheral leukocytes to produce IFN. IFN production was apparent by 10 h and peaked 24 h after exposure. Dose-response experiments indicated that IFN could be detected in cultures treated with concentrations of M protein as low as 6 μg/ml, whereas maximum IFN production occurred at a concentration of 200 μg/ml. The IFN had antigenic and physicochemical characteristics of IFN-γ. Preliminary leukocyte fractionation studies revealed that the IFN-producing cell was a nonadherent lymphocyte with receptors for sheep erythrocytes (T cell). Rabbit antisera specific for these structurally defined polypeptide fragments of streptococcal M protein (pep M5, pep M6, and pep M24) blocked IFN induction by each of the polypeptides. The data suggest that the different serotypes of streptococcal M protein may induce IFN by a common structural determinant shared by each of the polypeptide fragments tested.

UR - http://www.scopus.com/inward/record.url?scp=0021331491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021331491&partnerID=8YFLogxK

M3 - Article

C2 - 6418655

AN - SCOPUS:0021331491

VL - 43

SP - 122

EP - 126

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 1

ER -