Induction of macrophage killing of Leishmania donovani by human CD4+ T cell clones

P. C. Melby, G. Valencia-Pacheco, F. Andrade-Narvaez

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Immunity in leishmaniasis is mediated by T cells, but protective responses in humans have not been fully defined. In this study, the functional activity of CD4+ T cell clones derived from an immune individual was investigated to identify potentially protective responses. The T cells proliferated and produced interferon-gamma (IFN-γ) in response to a soluble Leishmania donovani antigen extract and live amastigotes. There was considerable variation in the anti-leishmanial activity of the T cell clones when they were co-cultured with L. donovani infected monocytes isolated from an HLA-DR,DQ matched donor. All of the clones which demonstrated antigen specific reactivity by proliferation or cytokine production induced some degree of inhibition of intracellular parasite replication, but only a few of the clones induced pronounced leishmanicidal activity. There was strong correlation between the level of amastigote-induced IFN-γ secretion and anti-leishmanial activity. This approach enables the identification of potentially protective immune responses in humans at the clonal level, and offers a means for the identification of the relevant antigen(s).

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalArchives of Medical Research
Volume27
Issue number4
StatePublished - Dec 19 1996
Externally publishedYes

Keywords

  • Cell-mediated immunity
  • Interferon-gamma
  • Leishmania
  • Macrophages
  • T-cell clones

ASJC Scopus subject areas

  • Medicine(all)

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