Induction of particle polymorphism by Cucumber necrosis virus coat protein mutants in vivo

Kishore Kakani, Ron Reade, Umesh Katpally, Thomas Smith, D'Ann Rochon

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The Cucumber necrosis virus (CNV) particle is a T=3 icosahedron consisting of 180 identical coat protein (CP) subunits. Plants infected with wild-type CNV accumulate a high number of T=3 particles, but other particle forms have not been observed. Particle polymorphism in several T=3 icosahedral viruses has been observed in vitro following the removal of an extended N-terminal region of the CP subunit. In the case of CNV, we have recently described the structure of T=1 particles that accumulate in planta during infection by a CNV mutant (R1+2) in which a large portion of the N-terminal RNA binding domain (R-domain) has been deleted. In this report we further describe properties of this mutant and other CP mutants that produce polymorphic particles. The T=1 particles produced by R1+2 mutants were found to encapsidate a 1.9-kb RNA species as well as smaller RNA species that are similar to previously described CNV defective interfering RNAs. Other R-domain mutants were found to encapsidate a range of specifically sized less-than-full-length CNV RNAs. Mutation of a conserved proline residue in the arm domain near its junction with the shell domain also influenced T=1 particle formation. The proportion of polymorphic particles increased when the mutation was incorporated into R-domain deletion mutants. Our results suggest that both the R-domain and the arm play important roles in the formation of T=3 particles. In addition, the encapsidation of specific CNV RNA species by individual mutants indicates that the R-domain plays a role in the nature of CNV RNA encapsidated in particles.

Original languageEnglish (US)
Pages (from-to)1547-1557
Number of pages11
JournalJournal of Virology
Volume82
Issue number3
DOIs
StatePublished - Feb 2008
Externally publishedYes

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Cucumber necrosis virus
Cucumis sativus
Capsid Proteins
coat proteins
Necrosis
genetic polymorphism
RNA
Viruses
mutants
RNA Viruses
Protein Subunits
protein subunits
Defective Viruses
Mutation
Proline
mutation
Virion
Plantae

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of particle polymorphism by Cucumber necrosis virus coat protein mutants in vivo. / Kakani, Kishore; Reade, Ron; Katpally, Umesh; Smith, Thomas; Rochon, D'Ann.

In: Journal of Virology, Vol. 82, No. 3, 02.2008, p. 1547-1557.

Research output: Contribution to journalArticle

Kakani, Kishore ; Reade, Ron ; Katpally, Umesh ; Smith, Thomas ; Rochon, D'Ann. / Induction of particle polymorphism by Cucumber necrosis virus coat protein mutants in vivo. In: Journal of Virology. 2008 ; Vol. 82, No. 3. pp. 1547-1557.
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