Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays

R. L. Ullrich, B. Allen, W. Lee, L. C. Satterfield, M. C. Jernigan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

These studies have examined alterations in the in vitro growth properties of mammary epithelial cells isolated at 1, 4, and 16 weeks after in vivo irradiation with -137Cs γ rays or fission-spectrum neutrons. Altered in vitro growth potential was characterized by the proliferation of epithelial foci (EF) from irradiated animals under conditions in which mammary cells from nonexposed animals senesced. These EF were further characterized based on their ability to be subcultured. Both γ and neutron irradiation resulted in the appearance of cells capable of forming EF. Further, with increased time in situ between irradiation and cell isolation, the frequency of EF which were capable of being subcultured indefinitely (EF(s)) increased. Reducing the γ-ray dose rate resulted in fewer EF(s) while reducing the neutron dose rate resulted in increased frequencies of EF(s). These data confirm earlier observations following γ irradiation and show these cellular changes are also observed following neutron irradiation. In addition, these data indicate that changes in dose rate primarily influence the emergence of immortalized cell populations.

Original languageEnglish (US)
Pages (from-to)102-106
Number of pages5
JournalRadiation Research
Volume129
Issue number1
StatePublished - 1992

Fingerprint

Neutrons
breasts
rays
induction
Breast
epithelial cells
irradiation
Epithelial Cells
neutrons
neutron irradiation
dosage
Cell Separation
cells
Growth
animals
neutron spectra
fission
isolation
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Ullrich, R. L., Allen, B., Lee, W., Satterfield, L. C., & Jernigan, M. C. (1992). Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays. Radiation Research, 129(1), 102-106.

Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays. / Ullrich, R. L.; Allen, B.; Lee, W.; Satterfield, L. C.; Jernigan, M. C.

In: Radiation Research, Vol. 129, No. 1, 1992, p. 102-106.

Research output: Contribution to journalArticle

Ullrich, RL, Allen, B, Lee, W, Satterfield, LC & Jernigan, MC 1992, 'Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays', Radiation Research, vol. 129, no. 1, pp. 102-106.
Ullrich RL, Allen B, Lee W, Satterfield LC, Jernigan MC. Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays. Radiation Research. 1992;129(1):102-106.
Ullrich, R. L. ; Allen, B. ; Lee, W. ; Satterfield, L. C. ; Jernigan, M. C. / Induction of phenotypically altered mammary epithelial cells by neutrons and γ rays. In: Radiation Research. 1992 ; Vol. 129, No. 1. pp. 102-106.
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