Induction of triggering receptors of myeloid cell (TREM-1) expression in fetal membranes and higher concentration of soluble TREM-1 in amniotic fluid with spontaneous preterm birth

Ramkumar Menon, Stephen J. Fortunato

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To document triggering receptors of myeloid cells (TREM-1) expression in fetal membranes and to estimate soluble TREM-1 (sTREM-1) concentrations in the amniotic fluid (AF) from spontaneous preterm birth (PTB). Methods: Fetal membranes at term not in labor placed in an organ explant system were stimulated with lipopolysaccharide (LPS). Membranes were also collected from PTB with and without microbial invasion of the amniotic cavity (MIAC).TREM-1 expression and sTREM-1 concentration (in culture media and in AF from PTB) were documented using RT-PCR and ELISA, respectively. Results: LPS and preterm labor induced fetal membrane TREM-1 expression. Higher sTREM-1 concentration was documented in membranes stimulated with LPS compared with unstimulated controls. In AF,sTREM-1 concentration was higher in PTB than normal term deliveries. PTB with MIAC had higher sTREM-1 concentration compared with PTB with no MIAC. Conclusions: TREM-1 is inducible in amniochorion by LPS and preterm labor. Higher sTREM-1 in PTB and in cases with MIAC suggests a role for TREM in infection-associated PTB.

Original languageEnglish (US)
Pages (from-to)825-830
Number of pages6
JournalReproductive Sciences
Volume15
Issue number8
DOIs
StatePublished - Oct 2008
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Premature Birth
Myeloid Cells
Amniotic Fluid
Lipopolysaccharides
Premature Obstetric Labor
Membranes
Culture Media
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction

Keywords

  • Amniotic fluid
  • Cytokines
  • Fetal membranes
  • Inflammation
  • Prematurity
  • Preterm labor

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

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title = "Induction of triggering receptors of myeloid cell (TREM-1) expression in fetal membranes and higher concentration of soluble TREM-1 in amniotic fluid with spontaneous preterm birth",
abstract = "Objective: To document triggering receptors of myeloid cells (TREM-1) expression in fetal membranes and to estimate soluble TREM-1 (sTREM-1) concentrations in the amniotic fluid (AF) from spontaneous preterm birth (PTB). Methods: Fetal membranes at term not in labor placed in an organ explant system were stimulated with lipopolysaccharide (LPS). Membranes were also collected from PTB with and without microbial invasion of the amniotic cavity (MIAC).TREM-1 expression and sTREM-1 concentration (in culture media and in AF from PTB) were documented using RT-PCR and ELISA, respectively. Results: LPS and preterm labor induced fetal membrane TREM-1 expression. Higher sTREM-1 concentration was documented in membranes stimulated with LPS compared with unstimulated controls. In AF,sTREM-1 concentration was higher in PTB than normal term deliveries. PTB with MIAC had higher sTREM-1 concentration compared with PTB with no MIAC. Conclusions: TREM-1 is inducible in amniochorion by LPS and preterm labor. Higher sTREM-1 in PTB and in cases with MIAC suggests a role for TREM in infection-associated PTB.",
keywords = "Amniotic fluid, Cytokines, Fetal membranes, Inflammation, Prematurity, Preterm labor",
author = "Ramkumar Menon and Fortunato, {Stephen J.}",
year = "2008",
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T1 - Induction of triggering receptors of myeloid cell (TREM-1) expression in fetal membranes and higher concentration of soluble TREM-1 in amniotic fluid with spontaneous preterm birth

AU - Menon, Ramkumar

AU - Fortunato, Stephen J.

PY - 2008/10

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N2 - Objective: To document triggering receptors of myeloid cells (TREM-1) expression in fetal membranes and to estimate soluble TREM-1 (sTREM-1) concentrations in the amniotic fluid (AF) from spontaneous preterm birth (PTB). Methods: Fetal membranes at term not in labor placed in an organ explant system were stimulated with lipopolysaccharide (LPS). Membranes were also collected from PTB with and without microbial invasion of the amniotic cavity (MIAC).TREM-1 expression and sTREM-1 concentration (in culture media and in AF from PTB) were documented using RT-PCR and ELISA, respectively. Results: LPS and preterm labor induced fetal membrane TREM-1 expression. Higher sTREM-1 concentration was documented in membranes stimulated with LPS compared with unstimulated controls. In AF,sTREM-1 concentration was higher in PTB than normal term deliveries. PTB with MIAC had higher sTREM-1 concentration compared with PTB with no MIAC. Conclusions: TREM-1 is inducible in amniochorion by LPS and preterm labor. Higher sTREM-1 in PTB and in cases with MIAC suggests a role for TREM in infection-associated PTB.

AB - Objective: To document triggering receptors of myeloid cells (TREM-1) expression in fetal membranes and to estimate soluble TREM-1 (sTREM-1) concentrations in the amniotic fluid (AF) from spontaneous preterm birth (PTB). Methods: Fetal membranes at term not in labor placed in an organ explant system were stimulated with lipopolysaccharide (LPS). Membranes were also collected from PTB with and without microbial invasion of the amniotic cavity (MIAC).TREM-1 expression and sTREM-1 concentration (in culture media and in AF from PTB) were documented using RT-PCR and ELISA, respectively. Results: LPS and preterm labor induced fetal membrane TREM-1 expression. Higher sTREM-1 concentration was documented in membranes stimulated with LPS compared with unstimulated controls. In AF,sTREM-1 concentration was higher in PTB than normal term deliveries. PTB with MIAC had higher sTREM-1 concentration compared with PTB with no MIAC. Conclusions: TREM-1 is inducible in amniochorion by LPS and preterm labor. Higher sTREM-1 in PTB and in cases with MIAC suggests a role for TREM in infection-associated PTB.

KW - Amniotic fluid

KW - Cytokines

KW - Fetal membranes

KW - Inflammation

KW - Prematurity

KW - Preterm labor

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