Inefficient Bypass of an Abasic Site by DNA Polymerase η

Lajos Haracska, M. Todd Washington, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

DNA polymerase η (Polη) bypasses a cis-syn thymine-thymine dimer efficiently and accurately, and inactivation of Polη in humans results in the cancer-prone syndrome, the variant form of xeroderma pigmentosum. Also, Polη bypasses the 8-oxoguanine lesion efficiently by predominantly inserting a C opposite this lesion, and it bypasses the O 6-methylguanine lesion by inserting a C or a T. To further assess the range of DNA lesions tolerated by Polη, here we examine the bypass of an abasic site, a prototypical noninstructional lesion. Steady-state kinetic analyses show that both yeast and human Polη are very inefficient in both inserting a nucleotide opposite an abasic site and in extending from the nucleotide inserted. Hence, Polη bypasses this lesion extremely poorly. These results suggest that Polη requires the presence of template bases opposite both the incoming nucleotide and the primer terminus to catalyze efficient nucleotide incorporation.

Original languageEnglish (US)
Pages (from-to)6861-6866
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number9
DOIs
StatePublished - Mar 2 2001

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DNA-Directed DNA Polymerase
Nucleotides
Pyrimidine Dimers
Thymine
Yeast
Yeasts
Kinetics
DNA
Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inefficient Bypass of an Abasic Site by DNA Polymerase η. / Haracska, Lajos; Washington, M. Todd; Prakash, Satya; Prakash, Louise.

In: Journal of Biological Chemistry, Vol. 276, No. 9, 02.03.2001, p. 6861-6866.

Research output: Contribution to journalArticle

Haracska, Lajos ; Washington, M. Todd ; Prakash, Satya ; Prakash, Louise. / Inefficient Bypass of an Abasic Site by DNA Polymerase η. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 9. pp. 6861-6866.
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