Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium

Liqun Zhang, Alexander Bukreyev, Catherine I. Thompson, Brandy Watson, Mark E. Peeples, Peter L. Collins, Raymond J. Pickles

Research output: Contribution to journalArticle

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Abstract

We constructed a human recombinant parainfluenza virus type 3 (rPIV3) that expresses enhanced green fluorescent protein (GFP) and used this virus, rgPIV3, to characterize PIV3 infection of an established in vitro model of human pseudostratified mucociliary airway epithelium (HAE). The apical surface of HAE was highly susceptible to rgPIV3 infection, whereas only occasional cells were infected when virus was applied to the basolateral surface. Infection involved exclusively ciliated epithelial cells. There was little evidence of virus-mediated cytopathology and no spread of the virus beyond the ciliated cell types. Infection of ciliated cells by rgPIV3 was sensitive to a neuraminidase specific for α2-6-linked sialic acid residues, but not to a neuraminidase that cleaves α2-3- and α2-8-linked sialic acid residues. This provided evidence that rgPIV3 utilizes α2-6-linked sialic acid residues for initiating infection, a specificity also described for human influenza viruses. The PIV3 fusion (F) glycoprotein was trafficked exclusively to the apical surface of ciliated cells, which also was the site of release of progeny virus. F glycoprotein localized predominately to the membranes of the cilial shafts, suggesting that progeny viruses may bud from cilia per se. The polarized trafficking of F glycoprotein to the apical surface also likely restricts its interaction with neighboring cells and could account for the observed lack of cell-cell fusion. HAE derived from cystic fibrosis patients was not more susceptible to rgPIV3 infection but did exhibit limited spread of virus due to impaired movement of lumenal secretions due to compromised function of the cilia.

Original languageEnglish (US)
Pages (from-to)1113-1124
Number of pages12
JournalJournal of Virology
Volume79
Issue number2
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Human parainfluenza virus 3
epithelium
Epithelium
Viruses
viruses
N-Acetylneuraminic Acid
Infection
infection
sialic acids
Glycoproteins
Cilia
Neuraminidase
cells
glycoproteins
sialidase
biological resistance
cilia
Virus Release
Cell Fusion
Orthomyxoviridae

ASJC Scopus subject areas

  • Immunology

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Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium. / Zhang, Liqun; Bukreyev, Alexander; Thompson, Catherine I.; Watson, Brandy; Peeples, Mark E.; Collins, Peter L.; Pickles, Raymond J.

In: Journal of Virology, Vol. 79, No. 2, 01.2005, p. 1113-1124.

Research output: Contribution to journalArticle

Zhang, Liqun ; Bukreyev, Alexander ; Thompson, Catherine I. ; Watson, Brandy ; Peeples, Mark E. ; Collins, Peter L. ; Pickles, Raymond J. / Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium. In: Journal of Virology. 2005 ; Vol. 79, No. 2. pp. 1113-1124.
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