Infectivity and pathogenesis of iridescent virus type 22 in various insect hosts

R. B. Tesh, T. G. Andreadis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This paper reports the results of a series of laboratory experiments to determine the infectivity and pathogenesis of iridescent virus type 22 (IV 22) for six species of mosquitoes, phlebotomine sand flies and triatomid bugs. Following inoculation, IV 22 replicated in all of the species tested, without producing noticeable mortality within a 14 day observation period. Examination of the infected insects by immunofluorescence demonstrated large amounts of viral antigen in many different organs. Electron microscopy done on infected mosquitoes (Aedes aegypti) showed large numbers of virus particles within cells of the fat body, muscle tracheal and midgut epithelium. Virus replication in the mosquitoes was confined to host cell cytoplasm and was similar to that described in the natural blackfly (Simulium) host. Transovarial transmission of IV 22 could not be demonstraed in A. aegypti, and only a small percentage of mosquito larvae could be infected orally. Results of these experiments are compatible with observations of other iridescent viruses; IV 22 is highly infectious for a wide range of insects when introduced into their hemolymph, but it is not very infectious per os. These characteristics would appear to limit its value as a potential biocontrol agent for Diptera.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalArchives of Virology
Volume126
Issue number1-4
DOIs
StatePublished - Mar 1992
Externally publishedYes

Fingerprint

Iridovirus
Insects
Culicidae
Simuliidae
Psychodidae
Fat Body
Hemolymph
Viral Antigens
Aedes
Virus Replication
Diptera
Virion
Fluorescent Antibody Technique
Larva
Electron Microscopy
Cytoplasm
Epithelium
Observation
Muscles
Mortality

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Infectivity and pathogenesis of iridescent virus type 22 in various insect hosts. / Tesh, R. B.; Andreadis, T. G.

In: Archives of Virology, Vol. 126, No. 1-4, 03.1992, p. 57-65.

Research output: Contribution to journalArticle

Tesh, R. B. ; Andreadis, T. G. / Infectivity and pathogenesis of iridescent virus type 22 in various insect hosts. In: Archives of Virology. 1992 ; Vol. 126, No. 1-4. pp. 57-65.
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