Infectivity of Trypanosoma rangeli in a promonocytic mammalian cell line

Y. Osorio, Bruno Travi, G. I. Palma, N. G. Saravia

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The existence of an intracellular stage of Trypanosoma rangeli in the vertebrate host was evaluated by experimental infection of the U937 histiocytic cell line with the San Agustin strain and the Ub66-5b clone. The identity of the parasites at the beginning and end of the experiments was confirmed through biological behavior in the vector and mammal hosts, isoenzymes, polymerase chain reaction (PCR), and monoclonal antibodies. Infectivity to U937 cells of T. rangeli obtained from culture and salivary glands was evaluated under different experimental conditions. These included 34 C vs. 37 C, opsonized vs. nonopsonized parasites, and 2, 4, 6, 24, 48, and 72 hr of cell-parasite contact. Trypanosoma rangeli adopted a characteristic nondividing amastigote-like form within U937 cells, which was different in size (P = 0.001) from Trypanosoma cruzi amastigotes. Culture forms of T. rangeli were more infective than parasites from salivary glands (P = 0.049) but were less infective than T. cruzi (P = 0.0001). Variations in temperature (34-37 C) and complement opsonization did not affect infectivity. Viability of intracellular forms was confirmed by feeding Rhodnius prolixus with T. rangeli-infected cells. Resistance of T. rangeli to the intracellular milieu could be an important mechanism in producing chronic infections in mammals and in the infection of triatomines.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalJournal of Parasitology
Volume81
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Trypanosoma rangeli
infectivity
parasite
pathogenicity
cell lines
Cell Line
U937 Cells
Parasites
parasites
amastigotes
mammal
Trypanosoma cruzi
salivary glands
Salivary Glands
Mammals
Infection
Rhodnius
infection
cells
mammals

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Infectivity of Trypanosoma rangeli in a promonocytic mammalian cell line. / Osorio, Y.; Travi, Bruno; Palma, G. I.; Saravia, N. G.

In: Journal of Parasitology, Vol. 81, No. 5, 1995, p. 687-693.

Research output: Contribution to journalArticle

Osorio, Y. ; Travi, Bruno ; Palma, G. I. ; Saravia, N. G. / Infectivity of Trypanosoma rangeli in a promonocytic mammalian cell line. In: Journal of Parasitology. 1995 ; Vol. 81, No. 5. pp. 687-693.
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