Inflammation-induced changes in peripheral glutamate receptor populations

S. M. Carlton, R. E. Coggeshall

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

The ionotropic glutamate receptors N-methyl-D-aspartate (NMDA), α- amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) have been localized on subpopulations of unmyelinated and myelinated sensory axons in normal skin. Behavioral studies indicate that activation of these receptors results in nociceptive behaviors and contributes to inflammatory pain. The goal of the present study was to determine if these glutamate receptors might contribute to the peripheral hypersensitivity observed in inflammation. The major findings were that 48 h following complete Freund's adjuvant (CFA)-induced inflammation, the proportions of unmyelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 43% and 48%, respectively, in cutaneous nerves in the inflamed paw compared to 48%, 22% and 27%, respectively, in the non-inflamed paw. The proportions of myelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 61% and 43%, respectively, compared to 43%, 42% and 28%, respectively, in the non-inflamed hindpaw. These increases were all significant. These data indicate that the number of sensory axons containing ionotropic glutamate receptors increases during inflammation, and this may be a contributing factor to peripheral sensitization in inflammation.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalBrain Research
Volume820
Issue number1-2
DOIs
StatePublished - Feb 27 1999

Fingerprint

Glutamate Receptors
Axons
Inflammation
Ionotropic Glutamate Receptors
Kainic Acid Receptors
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Population
Skin
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Freund's Adjuvant
Kainic Acid
N-Methylaspartate
Hypersensitivity
Pain
Acids

Keywords

  • Immunohistochemistry
  • Nociception
  • Primary afferents
  • Sensory neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Inflammation-induced changes in peripheral glutamate receptor populations. / Carlton, S. M.; Coggeshall, R. E.

In: Brain Research, Vol. 820, No. 1-2, 27.02.1999, p. 63-70.

Research output: Contribution to journalArticle

Carlton, S. M. ; Coggeshall, R. E. / Inflammation-induced changes in peripheral glutamate receptor populations. In: Brain Research. 1999 ; Vol. 820, No. 1-2. pp. 63-70.
@article{5c7bfdb969af459daf7869d131a450fb,
title = "Inflammation-induced changes in peripheral glutamate receptor populations",
abstract = "The ionotropic glutamate receptors N-methyl-D-aspartate (NMDA), α- amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) have been localized on subpopulations of unmyelinated and myelinated sensory axons in normal skin. Behavioral studies indicate that activation of these receptors results in nociceptive behaviors and contributes to inflammatory pain. The goal of the present study was to determine if these glutamate receptors might contribute to the peripheral hypersensitivity observed in inflammation. The major findings were that 48 h following complete Freund's adjuvant (CFA)-induced inflammation, the proportions of unmyelinated axons labeled for NMDA, AMPA or KA receptors were 61{\%}, 43{\%} and 48{\%}, respectively, in cutaneous nerves in the inflamed paw compared to 48{\%}, 22{\%} and 27{\%}, respectively, in the non-inflamed paw. The proportions of myelinated axons labeled for NMDA, AMPA or KA receptors were 61{\%}, 61{\%} and 43{\%}, respectively, compared to 43{\%}, 42{\%} and 28{\%}, respectively, in the non-inflamed hindpaw. These increases were all significant. These data indicate that the number of sensory axons containing ionotropic glutamate receptors increases during inflammation, and this may be a contributing factor to peripheral sensitization in inflammation.",
keywords = "Immunohistochemistry, Nociception, Primary afferents, Sensory neurons",
author = "Carlton, {S. M.} and Coggeshall, {R. E.}",
year = "1999",
month = "2",
day = "27",
doi = "10.1016/S0006-8993(98)01328-6",
language = "English (US)",
volume = "820",
pages = "63--70",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Inflammation-induced changes in peripheral glutamate receptor populations

AU - Carlton, S. M.

AU - Coggeshall, R. E.

PY - 1999/2/27

Y1 - 1999/2/27

N2 - The ionotropic glutamate receptors N-methyl-D-aspartate (NMDA), α- amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) have been localized on subpopulations of unmyelinated and myelinated sensory axons in normal skin. Behavioral studies indicate that activation of these receptors results in nociceptive behaviors and contributes to inflammatory pain. The goal of the present study was to determine if these glutamate receptors might contribute to the peripheral hypersensitivity observed in inflammation. The major findings were that 48 h following complete Freund's adjuvant (CFA)-induced inflammation, the proportions of unmyelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 43% and 48%, respectively, in cutaneous nerves in the inflamed paw compared to 48%, 22% and 27%, respectively, in the non-inflamed paw. The proportions of myelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 61% and 43%, respectively, compared to 43%, 42% and 28%, respectively, in the non-inflamed hindpaw. These increases were all significant. These data indicate that the number of sensory axons containing ionotropic glutamate receptors increases during inflammation, and this may be a contributing factor to peripheral sensitization in inflammation.

AB - The ionotropic glutamate receptors N-methyl-D-aspartate (NMDA), α- amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and kainate (KA) have been localized on subpopulations of unmyelinated and myelinated sensory axons in normal skin. Behavioral studies indicate that activation of these receptors results in nociceptive behaviors and contributes to inflammatory pain. The goal of the present study was to determine if these glutamate receptors might contribute to the peripheral hypersensitivity observed in inflammation. The major findings were that 48 h following complete Freund's adjuvant (CFA)-induced inflammation, the proportions of unmyelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 43% and 48%, respectively, in cutaneous nerves in the inflamed paw compared to 48%, 22% and 27%, respectively, in the non-inflamed paw. The proportions of myelinated axons labeled for NMDA, AMPA or KA receptors were 61%, 61% and 43%, respectively, compared to 43%, 42% and 28%, respectively, in the non-inflamed hindpaw. These increases were all significant. These data indicate that the number of sensory axons containing ionotropic glutamate receptors increases during inflammation, and this may be a contributing factor to peripheral sensitization in inflammation.

KW - Immunohistochemistry

KW - Nociception

KW - Primary afferents

KW - Sensory neurons

UR - http://www.scopus.com/inward/record.url?scp=0033608399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033608399&partnerID=8YFLogxK

U2 - 10.1016/S0006-8993(98)01328-6

DO - 10.1016/S0006-8993(98)01328-6

M3 - Article

C2 - 10023031

AN - SCOPUS:0033608399

VL - 820

SP - 63

EP - 70

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -