Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1

James E. Sligh, Christie M. Ballantyne, Susan S. Rich, Hal K. Hawkins, C. Wayne Smith, Allan Bradley, Arthur L. Beaudet

Research output: Contribution to journalArticle

505 Citations (Scopus)

Abstract

Gene targeting was used to produce mice deficient in intercellular adhesion molecule 1 (ICAM-1) or CD54, an immunoglobulin-like cell adhesion molecule that binds β2 integrins. Homozygous deficient animals develop normally, are fertile, and have a moderate granulocytosis. The nature of the mutation, RNA analysis, and immunostaining are consistent with complete loss of surface expression of ICAM-1. Deficient mice exhibit prominent abnormalities of inflammatory responses including impaired neutrophil emigration in response to chemical peritonitis and decreased contact hypersensitivity to 2,4-dinitrofluorobenzene. Mutant cells provided negligible stimulation in the mixed lymphocyte reaction, although they proliferated normally as responder cells. These mutant animals will be extremely valuable for examining the role of ICAM-1 and its counterreceptors in inflammatory disease processes and atherosclerosis.

Original languageEnglish
Pages (from-to)8529-8533
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
StatePublished - Sep 15 1993
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Dinitrofluorobenzene
Mixed Lymphocyte Culture Test
Gene Targeting
Contact Dermatitis
Emigration and Immigration
Cell Adhesion Molecules
Peritonitis
Integrins
Immunoglobulins
Atherosclerosis
Neutrophils
RNA
Mutation

Keywords

  • Cell adhesion
  • Gene targeting
  • Homologous recombination
  • Immune deficiency
  • Lymphocyte interactions

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Sligh, J. E., Ballantyne, C. M., Rich, S. S., Hawkins, H. K., Smith, C. W., Bradley, A., & Beaudet, A. L. (1993). Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. Proceedings of the National Academy of Sciences of the United States of America, 90(18), 8529-8533.

Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. / Sligh, James E.; Ballantyne, Christie M.; Rich, Susan S.; Hawkins, Hal K.; Smith, C. Wayne; Bradley, Allan; Beaudet, Arthur L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 18, 15.09.1993, p. 8529-8533.

Research output: Contribution to journalArticle

Sligh, JE, Ballantyne, CM, Rich, SS, Hawkins, HK, Smith, CW, Bradley, A & Beaudet, AL 1993, 'Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1', Proceedings of the National Academy of Sciences of the United States of America, vol. 90, no. 18, pp. 8529-8533.
Sligh, James E. ; Ballantyne, Christie M. ; Rich, Susan S. ; Hawkins, Hal K. ; Smith, C. Wayne ; Bradley, Allan ; Beaudet, Arthur L. / Inflammatory and immune responses are impaired in mice deficient in intercellular adhesion molecule 1. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 18. pp. 8529-8533.
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