Inflammatory and redox responses to ischaemia/reperfusion in human muscle

Ruksana Huda, Daneshvari R. Solanki, Mali Mathru

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The objective of this study was to identify cellular and plasma marker(s) of post-I/R (ischaemia/ reperfusion) in patients undergoing elective knee surgery where a tourniquet was used to facilitate a bloodless surgical field. We evaluated the inflammatory and redox response by measuring the mRNA levels of ICAM-1 (intercellular cell-adhesion molecule-1), MnSOD (manganese superoxide dismutase), GST-μ (glutathione transferase-μ) and Cu/ZnSOD (copper/zinc superoxide dismutase) in the operated muscle and blood cells pre-operatively (pre-tourniquet) and at various times after reperfusion (tourniquet release). We also measured plasma concentrations of IL (interleukin)-6, IL-8, sICAM-1 (soluble ICAM-1), IL-1/3 and TNF-α (tumour necrosis factor-α) using ELISA. Our results show a strong induction of MnSOD and GST-μ in granulocytes (but not in mononuclear cells or muscle) after reperfusion (2 and 4 h). There was no change in the mRNA level of Cu/ZnSOD after reperfusion. An up-regulation of membrane ICAM-1 in muscle and a decrease in sICAM-1 in plasma were detected after reperfusion. Plasma IL-6 and IL-8 levels (but not TNF-α or IL-1β) increased significantly over baseline at 2 and 4 h after reperfusion. Elevated expression of ICAM-1 in muscle, MnSOD and GST-μ in granulocytes and increased levels of plasma IL-6 and IL-8 may be considered as phase- and cell-specific markers of post-I/R of skeletal muscle in humans.

Original languageEnglish (US)
Pages (from-to)497-503
Number of pages7
JournalClinical Science
Volume107
Issue number5
DOIs
StatePublished - Nov 2004

Fingerprint

Reperfusion
Oxidation-Reduction
Intercellular Adhesion Molecule-1
Ischemia
Cell Adhesion Molecules
Muscles
Tourniquets
Superoxide Dismutase
Interleukin-8
Interleukin-6
Interleukin-1
Granulocytes
Muscle Cells
Tumor Necrosis Factor-alpha
Messenger RNA
Interleukin-3
Glutathione Transferase
Zinc
Copper
Blood Cells

Keywords

  • Human skeletal muscle
  • Inflammation
  • Intercellular cell-adhesion molecule-1 (ICAM-1)
  • Ischaemia/reperfusion
  • Manganese superoxide dismutase (MnSOD)
  • Tourniquet

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Inflammatory and redox responses to ischaemia/reperfusion in human muscle. / Huda, Ruksana; Solanki, Daneshvari R.; Mathru, Mali.

In: Clinical Science, Vol. 107, No. 5, 11.2004, p. 497-503.

Research output: Contribution to journalArticle

Huda, Ruksana ; Solanki, Daneshvari R. ; Mathru, Mali. / Inflammatory and redox responses to ischaemia/reperfusion in human muscle. In: Clinical Science. 2004 ; Vol. 107, No. 5. pp. 497-503.
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